芳樟醇体外抗红色毛癣菌活性及机理研究

Study on the activity and mechanism of linalool against Trichophyton rubrum in vitro

目的 探究芳樟醇抗红色毛癣菌的作用及机理。方法 采用改良CLSI M38-A2法测定芳樟醇抗红色毛癣菌的最小抑菌浓度;显微观察芳樟醇对红色毛癣菌的孢子萌发、菌丝形态的影响;电导率法判定芳樟醇对红色毛癣菌细胞膜的作用,并利用紫外分光光度分析芳樟醇对红色毛癣菌麦角甾醇合成的影响。透射电镜观察芳樟醇作用红色毛癣菌后其微观结构的改变。通过构建红色毛癣菌感染豚鼠模型,以评价芳樟醇对皮肤癣病的治疗效果。结果 芳樟醇抗红色毛癣菌的最小抑菌浓度为320μg·mL^(-1);芳樟醇可抑制红色毛癣菌的孢子萌发及菌丝的径向生长并导致细胞膜通透性增加以及抑制细胞膜中麦角甾醇的合成。显微观察结果显示细胞壁被降解,表面模糊、细胞内膜皱缩、细胞内容物泄漏。动物实验表明,芳樟醇可明显控制皮肤癣病的进程,改善豚鼠组织损伤程度。结论 芳樟醇具有抑制红色毛癣菌的活性,通过抑制其孢子萌发、菌丝生长,以及降解细胞壁、破坏细胞膜的完整性和抑制麦角甾醇合成等方式抑制红色毛癣菌的生长。

Objective The purpose of the research is to explore the effect and mechanism of linalool against Trichophyton rubrum.Methods The modified CLSI M38-A2 method was used to determine the minimum inhibitory concentration of linalool against Trichophyton rubrum.The effect of alcohol on the cell membrane of Trichophyton rubrum,and the effect of linalool on the synthesis of ergosterol in Trichophyton rubrum was analyzed by UV spectrophotometry.The change of microstructure of Trichophyton rubrum after linalool was observed by transmission electron microscope.To evaluate the therapeutic effect of linalool on dermatophytosis by constructing a guinea pig model infected with Trichophyton rubrum.Results The minimum inhibitory concentration of linalool against Trichophyton rubrum was 320 μg·mL^(-1);linalool could inhibit the spore germination and the radial growth of hyphae of Trichophyton rubrum,increase the cell membrane permeability and inhibit the synthesis of ergosterol in cell membranes.Microscopic observations revealed that the cell wall was degraded,the surface was blurred,the inner cell membrane shrunken,and the cell contents leaked.Animal experiments showed that linalool could significantly control the process of dermatophytosis and improve the degree of tissue damage in guinea pigs.Conclusion Linalool has the activity of inhibiting Trichophyton rubrum.It inhibits the growth of Trichophyton rubrum by inhibiting its spore germination,mycelial growth,degrading the cell wall,destroying the integrity of the cell membrane and inhibiting the synthesis of ergosterol.