基于生物网络探究愈溃凉血颗粒治疗溃疡性结肠炎的作用机制

Mechanism of Yukui Liangxue Granule in the Treatment of Ulcerative Colitis Based on Biological Network

目的 基于网络药理学方法探究愈溃凉血颗粒(yu kui granules, YKG)治疗溃疡性结肠炎(ulcerative colitis, UC)的生物学基础。方法 通过中药系统药理数据库及分析平台TCMSP数据库获取YKG的有效化合物及对应靶点,通过人类基因GeneCards数据库获得UC的相关靶点,并获取YKG与UC的共同靶点,对靶点之间的相互作用网络进行探究,并通过DAVID 6.8数据库对中药和UC的共同靶点进行GO生物学分析和KEGG通路富集分析。结果 共获得包括丁子香萜、槲皮素、β-谷甾醇、豆甾醇等187个有效化合物,抑癌基因P53(tumor protein p53, TP53)、转录因子和蛋白(JUN protein, JUN)、前列腺素内过氧化物合酶(prostaglandin endoperoxide synthase 2,PTGS2)、肿瘤坏死因子(tumor necrosis factor, TNF)、基质金属蛋白酶2(matrix metalloproteinase 2,MMP2)、表皮生长因子受体(epidermal growth factor receptor, EGFR)、白介素2(interleukin 2, IL-2)等90个共同靶点,经GO富集分析得到199个条目(P<0.01),经KEGG富集分析得到76条相关通路(P<0.01),其中主要与磷脂酰肌醇3激酶(phosphatidylinositol-3-kinase, PI3K)/蛋白激酶B(protein kinases B, Akt)信号通路、TNF信号通路、低氧诱导因子-1(hypoxia-inducible factor-1, HIF-1)信号通路密切相关。结论:YKG的多成分、多靶点、多通路的特点是治疗UC的关键基础。

Objective: To explore the biological basis of Yukui Liangxue Granule(YKG) in treating ulcerative colitis(UC)based on network pharmacology. Methods: The effective compounds and targets of YKG were obtained by TCMSP database, the related targets of UC were obtained by Gene Cards database, the common targets of YKG and UC were obtained, and the interaction network of the common targets was analyzed by DAVID 6.8 database for GO biological process analysis and KEGG pathway enrichment analysis. Results: A total of 187 effective compounds including mairin, quercetin, β-sitosterol, stigmasterol. 90 common targets including tumor suppressor gene p53(TP53), transcription factor and protein(JUN), prostaglandin endoperoxide synthase(PTGS2), tumor necrosis factor(TNF), matrix metalloproteinase 2(MMP2), epidermal growth factor receptor(EGFR), interleukin 2(IL-2) were obtained.199 items were obtained by GO enrichment analysis(P<0.01), and 76 related pathways were obtained by KEGG enrichment analysis(P<0.01), which were closely related to phosphatidylinositol 3 kinase(PI3 K)/protein kinase B(Akt) signal pathway, tumor necrosis factor(TNF) signal pathway and hypoxia inducible factor-1(HIF-1) signal pathway. Conclusion: YKG has the characteristics of multi-components, multi-targets and multi-pathways, which is the key basis for the treatment of UC.