正交试验法优选水药八角莲的姜制工艺

Optimization of Processing Technology for Dysosma Versipellis Grown in Guizhou with Ginger by Orthogonal tests

为了优选水药八角莲姜制的炮制工艺,通过L_(9)(3^(4))正交试验考察了姜用量、闷润时间、炒制时间3个因素的影响。采用HPLC法测定了4种成分(鬼臼毒素、4′-去甲基鬼臼毒素、槲皮素、山奈酚)的含量,优选出八角莲姜制的炮制工艺。结果表明,八角莲姜制的最佳炮制工艺为:姜用量5%,闷润时间60 min,炒制时间12 min。优选的八角莲姜制工艺重现性好,为八角莲后期进行炮制减毒增效研究提供了参考。

To research the best processing method for Dysosma Versipellisby Orthogonal tests.The orthogonal tests included the ginger amount,baked time and stuffy time as the influencing factors,an HPLC method was used for the determination of 4 ingredients(4′-Demethyl podophyllotoxin,quercetin,kaempferol,podophyllotoxin).The best processing technology of ginger Dysosma Versipellis was as follows:took a certain amount of Dysosma Versipellis,added 5%ginger to moisten 60 minutes,frying time was 12 minutes.This optimized technology was stable,feasible and reproducible.