Pim-3、p-S6、STAT-3在脂肪肉瘤的表达与临床病理特征及预后的关系

Relationship between expression of Pim-3,p-S6 and STAT-3 in liposarcoma with clinicopathological characteristics and prognosis

目的考察Pim-3、磷酸化S6核糖体蛋白(p-S6)、信号转导子和转录激活子3(STAT-3)在脂肪肉瘤中的表达及其与临床病理特征及预后的关系。方法收集该院病理科2009-2016年收治的42例确诊为脂肪肉瘤患者的肉瘤和瘤旁组织。采用免疫组织化学检测Pim-3、p-S6、STAT-3在肉瘤和瘤旁组织的表达,分析Pim-3、p-S6、STAT-3表达与脂肪肉瘤临床病理参数的关系,分析Pim-3、p-S6、STAT-3表达与脂肪肉瘤预后的关系。结果Pim-3、p-S6和STAT-3蛋白表达主要位于脂肪肉瘤细胞核,Pim-3、p-S6和STAT-3蛋白在脂肪肉瘤组织中的阳性表达分别为21例(50.00%)、19例(45.24%)和22例(52.38%),在瘤旁组织中的阳性表达分别为10例(23.81%)、8例(19.05%)和9例(21.43%),与瘤旁组织比较,脂肪肉瘤组织Pim-3、p-S6和STAT-3蛋白阳性表达率较高,差异有统计学意义(P<0.05)。不同肉瘤部位、TNM分期、病理类型、局部复发及远处转移的Pim-3、p-S6、STAT-3表达差异有统计学意义(P<0.05)。随访脂肪肉瘤患者至少5年,42例患者均获得随访。生存25例(59.52%),死亡17例(40.48%),均为脂肪肉瘤复发或恶化引起。Pim-3、p-S6和STAT-3阳性患者死亡率均分别高于Pim-3、p-S6和STAT-3阴性患者(P<0.05)。结论Pim-3、p-S6、STAT-3在脂肪肉瘤均呈异常表达,Pim-3、p-S6、STAT-3表达与肉瘤部位、TNM分期、病理类型、局部复发及远处转移相关,也与预后不佳相关。

Objective To investigate the expression of Pim-3,phospho-S6(p-S6),signal transducer and activator of transcription 3(STAT-3)in liposarcoma and its relationship with clinicopathological characteristics and prognosis.Methods The liposarcoma and parasarcoma tissues of 42 patients definitely diagnosed as liposarcoma admitted and treated in this hospital during 2009-2016 were collected.The Pim-3,p-S6 and STAT-3 expressions in the liposarcoma and parasarcoma tissues were detected by immunohistochemistry.The relationship between the expression of Pim-3,p-S6 and STAT-3 with the clinicopathological parameters of liposarcoma was analyzed.The relationship between the expression of Pim-3,p-S6 and STAT-3 with the prognosis of liposarcoma was analyzed.Results The expressions of Pim-3,p-S6 and STAT-3 proteins were mainly located in the nucleus of sarcoma cells.The positive expression of Pim-3,p-S6 and STAT-3 proteins in liposarcoma tissues were in 21 cases(50.00%),19 cases(45.24%)and 22 cases(52.38%),respectively.The positive expression in parasarcoma tissues were in 10 cases(23.81%),8 cases(19.05%)and 9 cases(21.43%),respectively.Compared with parasarcoma tissues,the positive expression rates of Pim-3,p-S6 and STAT-3 protein in liposarcoma tissue were higher,and the difference was statistically significant(P<0.05).The differences in the expressions of Pim-3,p-S6 and STAT-3 in different liposarcoma sites,TNM stages,pathological types,local recurrence and distant metastasis had statistical significance(P<0.05).The patients with liposarcoma were followed up for at least 5 years,and all 42 cases obtained followed up.Twenty-five cases(59.52%)survived and 17 cases(40.48%)died,all of them were caused by the recurrence or deterioration of liposarcoma.The death rate of the patients with Pim-3,p-S6 and STAT-3 positive was higher than that of the patients with Pim-3,p-S6 and STAT-3 negative(P<0.05).Conclusion Pim-3,p-S6 and STAT-3 are all abnormally expressed in liposarcoma.The expressions of Pim-3,p-S6 and STAT-3 are correlated to different liposarcoma sites,TNM stages,pathological types,local recurrence and distant metastasis,and also are correlated with the poor prognosis.