联合检测血浆Septin9基因甲基化及红细胞分布宽度对结直肠癌的诊断价值

Diagnostic value of combined detection of plasma Septin9 gene methylation and red blood cell distribution width in colorectal cancer

目的分析血浆Septin9基因甲基化(mSEPT9)联合红细胞分布宽度(RDW)检测对结直肠癌(CRC)的诊断价值。方法选取2020年6月至2022年6月该院收治的诊断为原发性CRC且术前未行放、化疗患者144例作为CRC组,选取同期内镜切除诊断为结直肠良性病变(腺瘤和增生性息肉)患者134例作为结直肠息肉组,选取同期100例健康体检者作为健康对照组。3组研究对象均接受mSEPT9及血常规检测,回顾性分析临床病理特征,采用受试者工作特征(ROC)曲线分析mSEPT9、RDW联合检测对CRC的诊断价值。结果CRC患者mSEPT9阳性率、RDW表达水平均明显高于结直肠息肉组和健康对照组,差异均有统计学意义(P<0.05)。CRC患者mSEPT9阳性率与TNM分期、T分期、M分期、肿瘤大小明显相关,RDW表达水平与TNM分期、T分期、肿瘤部位密切相关。RDW联合mSEPT9检测诊断CRC的ROC曲线下面积为0.794,差异有统计学意义(P<0.05),灵敏度为73.61%,特异度为83.00%。结论CRC患者血浆mSEPT9阳性率、RDW表达水平均明显增高,与TNM分期相关,二者联合检测可显著提高CRC诊断效能。

Objective To investigate the diagnostic value of methylated Septin9 DNA(mSEPT9)combined with red blood cell distribution width(RDW)in colorectal cancer(CRC).Methods One hundred and forty-four patients with primary CRC without preoperative radiochemotherapy treated in this hospital from June 2020 to June 2022 were selected as the CRC group.Contemporaneous 134 patients with colorectal benign lesions(adenomas and hyperplastic polyps)diagnosed by endoscopic resection were selected as the colorectal polyp group.The 100 healthy subjects undergoing the physical examination at the same period were selected as the healthy control group.The subjects in 3 groups received the mSEPT9 and blood routine detection.Their clinicopathological characteristics were retrospectively analyzed.The receiver operating characteristic(ROC)curve was applied to analyze the diagnostic value of mSEPT9 and RDW combined detection in CRC.Results The positive rate of mSEPT9 and RDW expression level in the CRC group were significantly higher than those in the colorectal polyp group and healthy control group,and the differences were statistically significant(P<0.05).The mSEPT9 positive rate in the patients with CRC was significantly correlated with the TNM stage,T stage,M stage and tumor size,and the RDW expression level was closely correlated with the TNM stage,T stage and tumor site.The area under the ROC curve of mSEPT9 and RDW combined detection was 0.794,and the difference was statistically significant(P<0.05),the sensitivity was 73.61%and the specificity was 83.00%.Conclusion The plasma mSEPT9 positive rate and RDW expression level in CRC patients are significantly increased,which is correlated with the TNM stage,their combined detection could significantly increase the CRC diagnostic efficiency.