喜马拉雅土壤酵母的分离及其发酵滤液对皮肤细胞功效的初步研究

Isolation of Himalayan soil yeast and efficacy of its fermentation filtrate on skin cell

从喜马拉雅地区植物龙胆的根际附近土壤中分离筛选出一株酿酒酵母Saccharomyces cerevisiae SC03,并对其发酵滤液进行皮肤细胞相关功效的研究,旨在为喜马拉雅地区微生物资源在化妆品领域的应用提供参考。通过收集西藏林芝龙胆根际土壤样品,分离挑选出较为理想的酵母菌株,对其进行鉴定和发酵培养,将发酵滤液添加至人皮肤成纤维细胞(FB)和角质形成细胞(KC)中。研究发现,喜马拉雅地区植物龙胆的根际附近土壤中分离筛选出的酵母菌株S.cerevisiae SC03,相对于模式酿酒酵母S288C共有199个结构变异片段,具有独特的基因组成。皮肤细胞活性研究表明,1%发酵滤液显著促进KC中Laminin332的合成,相对含量提高了81.16%;抑制UV辐照引起的ROS升高,KC中ROS含量降低了22.17%。2%的发酵滤液显著促进FB中DNA,ATP含量,分别提升了15.43%和12.89%;β-半乳糖苷酶阳性率显著下降了15.40%。结果表明,喜马拉雅地区分离的龙胆根际酵母SC03具有一定的抗氧化和抗衰老潜力,有望成为皮肤保湿、延缓皮肤衰老、增强皮肤屏障的化妆品活性物质使用,为其在化妆品生产中的开发利用提供数据支持。

This study aims to investigate the efficacy of the fermentation filtrate of Saccharomyces cerevisiae SC03 isolated from the rhizosphere soil of Gentiana scabra in Himalayan region on the human skin fibroblasts and keratinocytes.The results of this study also provide a reference for further promoting the application of microbial resources from the Himalayan region in the field of cosmetics.Soil yeast strains were isolated and screened from the rhizosphere soil samples collected from Nyingchi,Tibet,and the efficacy of the fermentation filtrate on the human skin fibroblasts and keratinocytes was explored.The results indicate that the genome of SC03 is very similar to that of the model yeast strain S.cerevisiae S288c,but also has special features.The fermentation filtrate of S.cerevisiae SC03 at a content of 0.5%-5%does not show cytotoxicity Himalaya Region to human skin fibroblasts aEnffdic akceyr oanti snkoinc ycteellss.The fermentation filtrate of S.cerevisiae SC03 at a content of 1%significantly promotes the synthesis of Laminin 332 in keratinocytes and inhibits the increase of ROS caused by UV irradiation,which is 81.16%and 22.17%,respectively.The fermentation filtrate of SC03 at a content of 2%significantly promotes DNA and ATP content of dermal fibroblasts and reduces cellular senescence-associatedβ-galactosidase positivity,which is 15.43%,12.89%,and 15.40%,respectively.These results suggest that the rhizosphere soil yeast fermentation filtrate has an effect of anti-oxidant,anti-aging and skin nutrition,which has the potential for further development and utilization in cosmetics production.