摘要
目的建立高效,稳定的超高效液相色谱-线性离子阱轨道阱串联质谱联用(UHPLC-LTQ-Orbitrap-MS/MS)方法,系统、全面地定性分析中药制剂保心宁胶囊中的化学成分组成。为保心宁胶囊质量控制方法的开发,标准的完善以及临床应用提供依据。方法采用UHPLC-LTQ-Orbitrap-MS/MS对保心宁胶囊中的化学成分进行快速分析。通过UHPLC-LTQ-Orbitrap-MS/MS扫描的到的化合物精确分子量,对照品和多级碎片离子信息,并结合相关文献以及裂解规律,实现对保心宁胶囊的准确定性分析。结果共鉴定出103种化学成分,包括萜类、皂苷类、酚酸类、黄酮类、有机酸类、香豆素类、苯酞类及姜黄素类等,并对化合物的药材归属进行了分类。结论本研究建立了对保心宁胶囊中化学成分的快速定性分析的方法,为保心宁胶囊的质量评价指标及药效物质基础研究提供参考。
OBJECTIVE To establish an efficient and stable ultra-high performance liquid chromatography linear ion trap orbital trap tandem mass spectrometry(UHPLC-LTQ-ORBITRAP-MS/MS)method for systematic and comprehensive qualitative analysis of chemical components in Baoxinning capsules,and provide a basis for the development of quality control methods,improvement of standards and clinical application of Baoxinning Capsules.METHODS The chemical components in Baoxinning capsules were analyzed by UHPLC-LTQ-ORBITRAP-MS/MS.The accurate molecular weight,reference substance and multistage fragment ion information of the compound were obtained by UHPLC-LTQ-ORBITRAP-MS/MS,combined with relevant literature and cracking law.RESULTS A total of 103 chemical constituents were identified,including terpenoids,saponins,phenolic acids,flavonoids,organic acids,coumarins,phthalides,curcumins and others.CONCLUSION A rapid qualitative analysis method of chemical components in Baoxinning capsules is established,and this study has a certain reference significance for the quality evaluation index and pharmacodynamic material basis of Baoxinning capsule.
作者
杜亚强
杨欢
谭春梅
陈碧莲
罗镭
DU Ya-qiang;YANG Huan;TAN Chun-mei;CHEN Bi-lian;LUO Lei(College of Pharmaceutical Sci-ence,Zhejiang Chinese Medical University,Hangzhou 310053,China;Zhejiang Institute for Food and Drug Control,Hangzhou 310052,China;NMPA Key Laboratory for Quality Evaluation of Traditional Chinese Medicine(Traditional Chinese Patent Medi-cine),Hangzhou 310052,China)
出处
《中国药学杂志》
CAS
CSCD
北大核心
2022年第21期1842-1850,共9页
Chinese Pharmaceutical Journal
基金
浙江省科技计划项目-药食同源功能健康食品开发-药食同源功能组分膜法制备及其功能健康食品全利用开发项目资助(2021C02019)。
