牛病毒性腹泻/黏膜病(1型+2型)、牛传染性鼻气管炎、牛副流感(3型)三联灭活疫苗(E2蛋白+C1株+HB01株)的研制与免疫效果评价

Development and Immune Effect Evaluation of Bovine Viral Diarrhea/Mucosal Disease(Type 1+Type 2),Infectious Bovine Rhinotracheitis and Bovine Parainfluenza(Type 3)Inactivated Vaccines(E2 Protein+C1 Strain+HB01 Strain)

采用CHO细胞表达牛病毒性腹泻/黏膜病病毒1型(BVDV1)E2蛋白,采用杆状病毒重组表达牛病毒性腹泻/黏膜病病毒2型(BVDV2)E2蛋白,采用MDBK细胞微载体悬浮培养技术培养牛传染性鼻气管炎病毒(IBRV)和牛副流感病毒3型(BPIV3),收获蛋白表达产物和细胞培养物,经纯化、灭活后与605佐剂混合,制备牛病毒性腹泻/黏膜病(1型+2型)、牛传染性鼻气管炎、牛副流感(3型)三联灭活疫苗(E2蛋白+C1株+HB01株)。将疫苗免疫健康易感牛进行免疫效果评价,结果表明该产品免疫效果良好,免疫牛IBRV和BPIV3中和抗体效价均可达到1∶77以上;BVDV1和BVDV2 E2蛋白琼扩抗体效价均可达到1∶32以上;免疫牛攻毒保护率均可达到4/5以上。

In this study,CHO cells were used to express bovine viral diarrhea/mucosal disease virus type 1(BVDV1)E2 protein,baculovirus recombinant was used to express bovine viral diarrhea/mucosal disease virus type 2(BVDV2)E2 protein,and MDBK cell microcarrier suspension culture technology was used to culture bovine infectious rhinotracheitis virus(IBRV)and bovine parainfluenza virus type 3(BPIV3).The protein expression products and virus cultures were harvested and mixed with 605 adjuvant after purification and inactivation to prepare inactivated vaccine of bovine viral diarrhea/mucosal disease(type 1+type 2),infectious bovine rhinotracheitis and bovine parainfluenza(type 3)(E2 protein+C1 strain+HB01 strain).The results showed that the immune effect was good,and the titers of IBRV and BPIV3 neutralizing antibodies of immunized cattle were more than 1∶77;The titers of BVDV1 and BVDV2 E2 protein antibody could reach more than 1∶32;The protection rate of immunized cattle against virus could reach more than 4/5.