葛根异黄酮对小鼠心肌损伤的保护作用及机制

Protective effect and mechanism of pueraria isoflavones on myocardial injury in mice

目的探索葛根异黄酮对小鼠心肌损伤的保护作用。方法小鼠分为正常组,模型组,葛根异黄酮低、中、高3个剂量实验组和普萘洛尔阳性组。模型组和给药组皮下注射异丙肾上腺素造模,第2 d开始灌胃葛根异黄酮和普萘洛尔。分光光度法检测心肌组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量和血清一氧化氮(NO)水平。结果正常组、模型组、低、中、高三个剂量实验组和阳性组SOD活性分别是(204.29±42.84),(124.25±17.60),(177.70±38.80),(193.92±36.05),(144.99±31.02)和(169.33±28.90)U·mg^(-1);这6组MDA的含量分别是(0.59±0.22),(2.12±1.34),(1.34±0.57),(1.14±0.28),(1.61±0.58)和(1.51±0.15)nmol·mg^(-1);这6组NO含量分别为(50.00±11.36),(18.18±4.45),(32.39±11.74),(45.91±8.58),(26.40±5.39)和(31.36±6.19)umol·L^(-1)。上述指标,模型组与正常组比较,差异均有统计学意义(均P<0.01),3个实验组与模型组比较,除高剂量组外,差异均有统计学意义(P<0.05或P<0.01)。结论葛根异黄酮对异丙肾上腺素所致的小鼠心肌损伤具有明显的保护作用,机制与其抗氧化损伤、舒张血管等因素有关。

Objective To investigate the protective effect of pueraria isoflavones on myocardial injury in mice.Methods Mice were divided into normal group,model group,pueraria isoflavone low,medium and high dose experimental groups and propranolol positive group.Model group and drug administration group were subcutaneously injected with isoproterenol(ISO)for membrane formation,and drug administration group began to give drugs by gavage on the next day.The contents of superoxide dismutase(SOD),malondialdehyde(MDA)and serum nitric oxide(NO)were measured by spectrophotometry.Results The SOD activity of normal group,model group,low,medium and high dose experimental groups and positive group were(204.29±42.84),(124.25±17.60),(177.70±38.80),(193.92±36.05),(144.99±31.02),(169.33±28.90)U·mg^(-1),respectively;the contents of MDA in the six groups were(0.59±0.22),(2.12±1.34),(1.34±0.57),(1.14±0.28),(1.61±0.58),(1.51±0.15)nmol·mg^(-1),respectively;the contents of NO in the six groups were(50.00±11.36),(18.18±4.45),(32.39±11.74),(45.91±8.58),(26.40±5.39),(31.36±6.19)μmol·L^(-1),respectively.Compared between the model group and the normal group,the difference of the above factors were significant(all P<0.01).Compared between experimental group and model group,the differences of the factors were significant except the high-dose group(P<0.05 or P<0.01).Conclusion Pueraria isoflavones have protective effects on myocardial injury induced by ISO in mice,and its mechanism may be related to antioxidant injury,vasodilation and other factors.