Nr-CWS促进间充质干细胞分泌生物活性更高的外泌体

Nr-CWS promotes MSC secretion of exosomes with higher biological activity

目的探索红色诺卡式菌细胞壁骨架(Nr-CWS)是否能够提高间充质干细胞外泌体的产量,增强间充质干细胞外泌体促进修复与再生的生物学活性。方法用红色诺卡式菌细胞壁骨架培养间充质干细胞,提取外泌体后,鉴定外泌体形态和表面标记蛋白,并测量外泌体蛋白含量,比较其产量变化。CCK-8细胞增殖实验和划痕实验检测外泌体促进人皮肤成纤维细胞的增殖和迁移,体外毛细血管生成实验检测外泌体促进人脐静脉血管内皮细胞再生的能力。结果红色诺卡式菌细胞壁骨架培养间充质干细胞可以极大提高间充质干细胞外泌体的蛋白含量,并且没有改变外泌体的经典形态和表面标记蛋白。此外,外泌体促进人皮肤成纤维细胞增殖和迁移的能力和促进人脐静脉血管内皮细胞血管再生的活性均明显增强。结论红色诺卡式菌细胞壁骨架极大提高了间充质干细胞外泌体的产量,提升其促进修复和再生的生物活性。

Objective To explore the production of exosomes secreted by mesenchymal stem cells and enhance their biological activity to promote healing and regeneration.Methods Mesenchymal stem cells were cultivated with Nocardia rubra cell wall skeleton and to extract exosomes.Then,the morphology of exosomes and surface marker proteins were identified and the amounts of exosomes were measured.The proliferation and migration of human dermal fibroblasts were detected by CCK-8 and scratch assays,and the capacity of exosomes to promote the regeneration of human umbilical vein endothelial cells was detected by in vitro angiogenesis assay.Results Cultivating mesenchymal stem cells with Nocardia rubra cell wall skeleton greatly increased the production of exosomes by mesenchymal stem cells,while the classical morphology of exosomes and surface marker proteins were not altered.Moreover,we detected the enhanced capacity of exosomes to promote the proliferation and migration of human dermal fibroblasts and the regenerationof human umbilical vein endothelial cells.Conclusions Nocardia rubra cell wall skeleton can improve the production of exosomes by mesenchymal stem cells and enhance their biological activity to promote healing and regeneration.