建兰“四季达摩”根状茎增殖分化及壮苗生根研究

The Proliferation and Differentiation of Rhizome and Rooting of Strong Seedling of Cymbidium ensifolium‘sijidamo’

以建兰“四季达摩”根状茎为材料,研究不同基本培养基、添加物对组培增殖分化以及不同糖浓度、生长调节剂种类和浓度对壮苗生根的影响。结果表明,在增殖阶段,花宝1号宜作为基本培养基,香蕉泥可使根状茎增粗;在分化阶段,适宜的根状茎分化培养基为花宝2号3 g/L+多维片1/4片+香蕉60 g/L+NAA 1.0 mg/L+6-BA 1.0 mg/L,多维片有利于芽的分化;在壮苗生根阶段,有利于组培壮苗生根的培养基为花宝1号3 g/L+60 g/L糖+1.0 mg/L NAA+10 mg/L多效唑,其假鳞茎健壮,根较粗。该研究以期提高种苗繁育速度和质量,为其优质高效快繁提供技术支撑。

Taking the rhizome as material,the effects of different basic media and additives on tissue culture proliferation and differentiation,as well as the effects of different sugar concentrations,types and concentrations of growth regulators on the rooting of strong seedlings were studied.The results showed that in the proliferation stage,Huabao No.1 should be used as the basic medium,and banana mud could thicken the rhizome.In the differentiation stage,the suitable medium for rhizome differentiation was Huabao No.23 g/L+Duowei tablet 1/4 piece+Banana 60 g/L+NAA 1.0 mg/L+6-BA 1.0 mg/L,Duowei tablet were conducive to axillary bud differentiation.In the strong seedling rooting stage,the suitable medium for the rooting of strong seedlings was Huabao No.13 g/L+60 g/L sugar+1.0 mg/L NAA+10 mg/L Paclobutrazol.The pseudobulbs were strong and its roots were thick.The study were focus on improving the speed and quality of seedling breeding and providing technical support for its high-quality,efficient and rapid propagation.