5株H9N2亚型禽流感病毒分离鉴定及HA基因的序列分析

Isolation and Identification of 5 Strains of H9N2 Subtype Avian Influenza Virus Sequence Analysis of HA Gene

[目的]对山东省部分地区疑似H9N2亚型禽流感病毒的5份病料样品进行鉴定,并确定其所属系谱。[方法]通过鸡胚接种、血清学试验、RT-PCR检测和基因测序等方法对样品进行分离、鉴定和分析。[结果]5个分离株与A/chicken/Guangxi/55/2005(H9N2)的同源性在90%以上,并属同一分支,与参考株A/chicken/Henan/G19/2011(H9N2)的同源性不到90.0%,与其亲缘关系最近的疫苗株A/chicken/Jiangsu/WJ57/2012(H9N2)的同源性在92.1~93.5%。[结论]分离的5个毒株均属于欧亚系Ⅰ群h9.4.2.5分支,且与参考株和疫苗株之间产生一定的遗传距离。

[Objective]The homology five samples of suspected H9N2 subtype avian influenza virus were collected from parts of Shandong Province,China,and their pedigree was determined.[Method]The samples were isolated,identified and analyzed by inoculation of chicken embryo,serological test,RT-PCR and gene sequencing.[Result]The homology of five isolates with A/chicken/Guangxi/55/2005(H9N2)was above 90%,belonged to the same branch,and that with the reference strains A/chicken/Henan/G19/2011(H9N2)was less than 90.0%,the homology with the most closely related vaccine strain A/chicken/Jiangsu/WJ57/2012(H9N2)was at about 92.1%-93.5%.[Conclusion]The five isolates belong to the Eurasian h9.4.2.5 branch departmentⅠgroup,produce certain genetic distance between the reference strain and the vaccine strain.