GDF9在PCOS患者成熟卵泡中的表达及其与内分泌激素的相关性

Expression of GDF9 in mature follicles and its correlations with hormones in polycystic ovary syndrome patients

目的:探讨卵源性因子生长分化因子9(GDF9)在多囊卵巢综合征(PCOS)患者控制性卵巢刺激促排周期成熟卵泡中的表达,及其与内分泌激素的相关性。方法:选取115例接受体外受精控制性卵巢刺激治疗不孕患者,包括PCOS患者61例和卵巢功能正常的对照组54例。于取卵日获取成熟卵泡的卵泡液和卵丘细胞。比较两组患者一般资料及月经周期第3~5天的基础内分泌激素情况。采用ELISA法检测两组患者的卵泡液GDF9蛋白浓度;免疫组化法检测其卵丘细胞上GDF9蛋白表达水平;Pearson相关分析评估GDF9表达与血清睾酮(T)、卵泡刺激素(FSH)、黄体生成素(LH)和抗缪勒管激素(AMH)水平的相关性。结果:PCOS患者成熟卵泡GDF9表达水平明显低于对照组(P<0.01),相关性分析显示PCOS患者卵泡液GDF9水平与T和FSH呈正相关;与LH、LH/FSH和AMH呈负相关(P<0.05)。而在对照组中,GDF9与T、FSH、LH、LH/FSH和AMH无显著相关性(P>0.05)。结论:GDF9在PCOS患者成熟卵泡中表达水平降低,并与血清T、FSH、LH、LH/FSH和AMH相关。

AIM:To assess the expression of growth differentiation factor 9(GDF9)in mature follicles of polycystic ovary syndrome(PCOS)patients undergoing controlled ovarian hyperstimulation,and to explore its relationship with hormones.METHODS:A total of 115 infertile patients undergoing controlled ovarian hyperstimulation for in vitro fertilizaion were enrolled in the study,including 61 PCOS patients and 54 patients with normal ovarian.We compared the anthropometric data and basic hormone levels between the two groups.Follicular fluid and cumulus cells of mature follicles were collected.ELISA was used to examine the level of GDF9 in follicular fluid.Immunohistochemical method was performed to detect GDF9 protein expression in cumulus cells.Pearson correlation analysis was used to evaluate the relationship between GDF9 and testosterone(T),follicle-stimulating hormone(FSH),luteinizing hormone(LH),LH/FSH and anti-Mullerian hormone(AMH).RESULTS:The GDF9 level in mature follicles of PCOS patients was significantly lower than that of the controls(P<0.01).Correlation analysis showed that GDF9 level in PCOS was positively correlated with T and FSH,and negatively correlated with LH,LH/FSH and AMH.There was no significant correlation of GDF9with serum hormones in control group.CONCLUSION:GDF9 level is reduced in mature follicles of PCOS patients and is closely related to T,FSH,LH,LH/FSH and AMH.