摘要
生命科学研究促进了组学技术的发展,除了基因组、转录组和蛋白组可以从基因和蛋白功能等方面揭示生命科学活动外,生物体内还存在着十分精细的调控体系,既有直接参与能量代谢的糖类、脂质及其中间代谢物,也有对新陈代谢起重要调节作用的物质,它们在体内形成相互关联的代谢网络,共同参与对生命活动的调控。
A massive amount of primary data for all low-molecular-weight metabolites in living organism was generated in cellular metabolomics,which provides the basis for early biological changes in cells caused by perturbation of metabolic pathways.In vitro study has many advantages such as less impact of external factors,controllable experimental conditions,high level of reproducibility,low cost and easy interpretation of results.Therefore,cell metabolomics research has been widely used in drug development and safety evaluation,etc.In order to obtain a more realistic and comprehensive metabolic profile,it is necessary to design a reasonable experimental protocol,such as controlling the medium source and cell growth cycle to reduce the difference in the metabolic profile,selecting appropriate cell processing and analysis methods to obtain comprehensive metabolite information.This review discusses the experimental process of cell metabolomics and its application in drug research,aiming to further understand the research and development of cellular metabolomics.
作者
王艳丽
刘春花
潘洁
孙佳
刘亭
李勇军
陆苑
WANG Yan-li;LIU Chun-hua;PAN Jie;SUN Jia;LIU Ting;LI Yong-jun;LU Yuan(Guizhou Provincial Key Laboratory of Pharmaceutics&State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550004,China;School of Pharmacy,Guizhou Medical University,Guiyang 550004,China;Engineering Research Center for the Development and Application of Ethnic Medicine and TCM(Ministry of Education),Guizhou Medical University,Guiyang 550004,China)
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2022年第12期2258-2267,共10页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.81860718)
贵州省科技厅科研项目(黔科合支撑[2021]一般421
黔科合基础-ZK[2022]一般374)
贵州省普通高等学校科技拔尖人才项目(No.KY[2021]033)
贵州省优秀青年科技人才项目(黔科合平台人才[2021]5619号)
2021年度贵州省卫生健康委科学技术基金资助项目(No.gzwkj2021-450)
贵州省教育厅青年科技人才成长项目(黔教合KY字[2021]171)。
关键词
细胞代谢组学
药物研发
研究流程
应用
Cell metabolomics
Drug development
Research process
Application