广西地区不同黄羽肉鸡品系种公鸡群中禽白血病流行病学调查

Epidemiological investigation of avian leukosis in breeder cocks from different yellow-feathered broilers flocks in Guangxi

禽白血病病毒(ALV)在我国黄羽肉鸡群中的流行仍较普遍,前期已证实公鸡及其精液在ALV传播中具有重要作用,为了解广西地区部分黄羽肉鸡品系的种公鸡群中ALV流行情况,本研究分别采用血浆和精液实施病毒分离对广西地区12个黄羽肉鸡品系的种公鸡群开展了流行病学调查和ALV的分离鉴定。结果显示,12个品系种公鸡群经血浆病毒分离阳性率分别为0.74%(1/135),3.46%(8/231),1.37%(1/73),3.78%(9/238),8.12%(38/468),5.33%(73/1370),5.45%(9/165),2.13%(1/47),0.00%(0/29),2.50%(1/40),1.72%(3/174),2.50%(5/200);精液病毒分离阳性率分别为0.74%(1/135),1.73%(4/231),1.37%(1/73),1.26%(3/238),1.71%(8/468),6.64%(91/1370),4.24%(7/165),0.00%(0/47),0.00%(0/29),0.00%(0/40),5.17%(9/174),1.50%(3/200)。对比发现同一种公鸡群血浆病毒分离和精液病毒分离结果并不一致,其中7个鸡群血浆病毒分离阳性率高于精液病毒分离阳性率,2个鸡群精液病毒分离阳性率高于血浆病毒分离阳性率,对其中4个鸡场的6株ALV分离株gp85基因测序分析显示其均为J亚群ALV。本研究结果表明,在广西地区12个黄羽肉鸡品系种公鸡群中存在ALV的流行,需要实施进一步的净化措施加以控制。

The prevalence of avian leukosis virus(ALV) in yellow-feathered broiler flocks is still quite common, it has been confirmed that cocks and the semen play an important role in ALV transmission.In order to understand the prevalence of ALV in yellow-feathered broilers flocks in Guangxi, the samples of plasma and semen were used for virus isolation and the isolation and subgroup identification of ALV were carried out from twelves yellow-feathered broiler flocks in Guangxi.The results showed that the positive rates of blood virus isolation from twelves yellow-feathered broiler cocks were 0.74%(1/135),3.46%(8/231),1.37%(1/73),3.78%(9/238),8.12%(38/468),5.33%(73/1370),5.45%(9/165),2.13%(1/47),0.00%(0/29),2.50%(1/40),1.72%(3/174),2.50%(5/200),respectively.The positive rates of semen virus isolation were 0.74%(1/135),1.73%(4/231),1.37%(1/73),1.26%(3/238),1.71%(8/468),6.64%(91/1370),4.24%(7/165),0.00%(0/47),0.00%(0/29),0.00%(0/40),5.17%(9/174),1.50%(3/200),respectively.Comparison indicated that the results of plasma virus isolation and semen virus isolation were not consistent from the same breeder flocks,the positive rate of plasma virus isolation was higher than that of semen virus isolation in seven chicken flocks,the positive rate of semen virus isolation was higher than that in plasma in two chicken flocks,the sequencing analysis of gp85 gene of six isolates from four chicken farms showed that all the isolates belonged to subgroup J in this study.The results showed that ALV is still prevalent in twelves yellow-feathered broiler flocks in Guangxi,and further eradication programs are needed to control ALV.