重组人促红细胞生成素对戊四氮致痫大鼠海马蛋白质组学的研究

Study of recombinant human erythropoietin on hippocampal proteomics in pentylenetetrazole-induced epileptic rats

目的 研究重组人促红细胞生成素(Recombinant human erythropoietin,r-HuEPO)对戊四氮(Pentetrazol,PTZ)致痫大鼠海马组织中差异表达的蛋白质,为探讨癫痫发病机制和寻找新的治疗靶点提供理论依据。方法 将12只体重为230~250 g的6~8周龄SD大鼠随机分为两组:PTZ组、PTZ+EPO组。运用基于质谱的TMT标记技术将r-HuEPO对PTZ致痫大鼠海马组织的差异蛋白进行分析鉴定。结果 检测到PTZ致痫大鼠海马组织中139个差异表达的蛋白质点,其中55个表达上调,84个表达下调。结论 r-HuEPO通过上调PTZ致痫大鼠海马组织中异柠檬酸脱氢酶(Isocitrate dehydrogenase,NADP)、还原型辅酶Ⅱ(Reduced nicotinamide purine dinucleotide phosphate,NADPH)、硫氧还蛋白还原酶(Thioredoxin reductase 2 mitochondrial,TrxR),减轻神经细胞损伤。

Objective To study the differentially expressed proteins of recombinant human erythropoietin(rHuEPO) in hippocampus of Pentetrazol(PTZ)-induced epileptic rats, and to provide a basis for exploring the pathogenesis of epilepsy and seeking new therapeutic targets. Methods Twelve 6~8-week-old Sprague Dawley rats weighing 230~250 g were randomly divided into two groups: PTZ group, PTZ+ EPO group. The differential proteins of recombinant human EPO in hippocampus of pentylenetetrazole-induced epileptic rats were analyzed and identified by TMT technique based on mass spectrometry. Results 139 differentially expressed protein sites were detected in hippocampal tissues of epileptic rats, of which 55 were up-regulated and 84 down-regulated. Conclusion Recombinant human erythropoietin can inhibit many differentially expressed proteins in the hippocampus of pentaerythraze-induced eclampsia rats by upregulation of Isocitrate dehydrogenase(NADP), Reduced nicotinamide purine dinucleotide phosphate(NADPH), Thioredoxin reductase 2 mitochondrial(TrxR), reduce nerve cell damage.