一种肿瘤类器官制备的改良方法及其初步的应用研究

An improved method and the preliminary application research for the preparation of organoids from tumor patients

目的:构建肿瘤类器官(patient derived organoids,PDO)平台,比较传统方法与改良方法之间的模型成功率。方法:建立经典肿瘤PDO模型,改良机械粉碎程度、消化时间、消化温度等条件后建立PDO模型,通过HE染色评价PDO与原始肿瘤标本的差异。结果:成功建立59例肿瘤PDO,涵盖17个肿瘤类型;HE染色结果显示,病人来源的PDO从形态、增殖性表达等方面与亲本高度相似。优化后的方法PDO细胞提取率明显提高、并且生长状况良好。结论:PDO保持了与亲本相似的特性,改良后的方法,其PDO模型成功率要显著高于传统方法。

Objective:To construct the platform of patient derived organs(PDO) and compare the success rate of the model between the traditional method and the improved method.Methods:The classic PDO model of tumor was established,and the improved PDO model was established after modifying the conditions such as mechanical crushing degree,digestion time and temperature.The difference between PDO and original tumor samples was evaluated by HE staining.Results:PDO was successfully established in 59 cases,covering 17 tumor types.The results of HE staining showed that PDO from patients was highly similar to the original tumors in morphology and proliferative expression.After optimization,the extraction rate of PDO cells was significantly improved,and the growth condition was satisfactory.Conclusion:PDO keeps the similar characteristics with its parents.The success rate of PDO model of the improved method is significantly higher than that of the traditional method.