两株野生木霉的鉴定及其产漆酶和纤维素酶研究

Identification of Two Wild Trichoderma Strains and Study on Laccase and Cellulase Production

利用微生物产生的活性酶系来降解纤维素、木质素等多聚物是资源绿色节能利用的主要趋势。该研究以在锡林郭勒地区分离的两株木霉菌XLGL201709099和XLGL201709112为研究对象,通过ITS序列分析对其进行鉴定,采用四种不同固体培养基,以菌丝的生长能力和最终生物量为评价指标,进行最优固体培养基的筛选;分别测定两株木霉菌株的总抗氧化能力(ABTS法)、滤纸酶活(FPA法)来评价其产漆酶和产纤维素酶的能力。结果表明,木霉菌株XLGL201709099为黄绿木霉(Trichoderma aureoviride),木生菌标准培养基(GPY)为其最优固体培养基,该木霉菌株产漆酶酶活为6.319 U/L,总纤维素酶活为0.538 U/g。木霉菌株XLGL201709112为康氏木霉(Trichoderma koningii),GPY培养基为其最优固体培养基,该木霉菌株产漆酶酶活为6.138 U/L,总纤维素酶活为0.888 U/g。通过对两株木霉的产漆酶和纤维素酶能力的研究,以期为利用木霉来降解本地区的纤维素、木质素等资源提供有益借鉴。

It is the main trend of green and energy saving utilization of resources to degrade cellulose, lignin and other polymers by using active enzyme system produced by microorganisms. In this study, XLGL201709099 and XLGL201709112 were isolated from Xilingol area and identified by ITS sequence analysis. Four different solid media were used, and the growth capacity and final biomass of mycelia were used as evaluation indexes to screen the optimal solid media. The total antioxidant capacity(ABTS) and filter paper enzyme activity(FPA) of two strains of trichoderma were determined respectively to evaluate the laccase and cellulase production capacity. The results showed that XLGL201709099 was the Trichoderma aureoviride strain, and GPY was the optimal solid medium. The laccase production activity of the strain was 6.319 U/L, and the total cellulase activity was 0.538 U/g.XLGL201709112 was the Trichoderma koningii strain, and GPY medium was the optimal solid medium. The laccase production activity of the strain was 6.138 U/L, and the total cellulase activity was 0.888 U/g. The laccase and cellulase production capacity of two strains of trichoderma were studied in order to provide useful reference for the degradation of cellulose and lignin in this area.