心房钠尿肽的抗大鼠运动病作用研究

Study on the anti-motion sickness effect of atrial natriuretic peptide in rats

目的通过旋转刺激和腹腔注射精氨酸加压素(AVP)的方法,建立两种运动病诱导模型,观察心房钠尿肽(ANP)的抗运动病作用。方法SD大鼠24只,按照实验设计分为实验组(n=12)和对照组(n=12)。对照组不作任何处理。实验组大鼠给予旋转刺激(120 min)诱导运动病发生后,取6只大鼠检测其0.15%糖精钠溶液的饮用量及高岭土食用量的变化,并与对照组对比分析大鼠运动病的敏感性;实验组另外6只大鼠在腹腔注射AVP,观察大鼠对0.15%糖精钠溶液的厌饮行为,然后观察ANP腹腔注射对大鼠旋转刺激及AVP使用后0.15%糖精钠溶液厌饮行为的抑制作用。结果旋转刺激诱导大鼠产生厌饮糖精钠溶液(味觉厌恶)及异嗜高岭土(异食癖)的行为(P<0.05或P<0.01);腹腔注射AVP后,亦可诱导大鼠产生厌饮糖精钠溶液的行为(P<0.05或P<0.01);而腹腔注射ANP后能有效地抑制旋转刺激或腹腔注射AVP引起的大鼠条件性味觉厌恶行为(P<0.05或P<0.01)。结论类似于旋转刺激,腹腔注射AVP也可以诱发大鼠运动病表现;腹腔注射ANP在这两种运动病模型上均具有拮抗大鼠运动病的作用。

Objective To study the anti-motion sickness effect of atrial natriuretic peptide(ANP)through establishing two types of motion sickness rat models by giving rotational stimulation or intraperitoneal arginine vasopressin(AVP)injection.Methods Normal Sprague-Dawley rats were rotated 120 min to induce motion sickness.The behavioural changes in their ingestion of 0.15% saccharin sodium solution(SSS)and consumption of kaolin were measured;and the susceptibilities of the rats to motion sickness were thus determined.The behavioural changes in the ingestion of 0.15%SSS after the intraperitoneal injection of AVP were also recorded.Finally,after the intraperitoneal injection of ANP,inhibitory influences on taste aversion to 0.15% SSS induced by these two methods were determined.Results After the rotational stimulation,the rats reduced their intake of 0.15% SSS(taste aversion)or increased the intake of kaolin(pica).The intraperitoneal injection of AVP in rats could also induce taste aversion to 0.15% SSS.However,the intraperitoneal injection of ANP effectively inhibited this conditioned taste aversion to 0.15% SSS induced either by rotational stimulation or AVP injection.Conclusion Both rotational stimulation and intraperitoneal injection of AVP could induce motion sickness;and the intraperitoneal injection of ANP could help relieve motion sickness in rats.