漂浮法和离心法富集棘球绦虫卵的优化研究

Study on the optimization of flotation and centrifugation methods for the enrichment of Echinococcus eggs

为了建立一种快速、高效富集棘球绦虫卵的方法,试验探讨了漂浮法和离心法富集棘球绦虫卵时的关键试验条件的设定,在漂浮法中,比较了使用蔗糖、CaCl_(2)、NaCl、MgSO_(4)、ZnCl_(2)、ZnSO_(4)的饱和溶液作为漂浮剂时的虫卵富集率,并筛选出最佳的漂浮时间(10,20,30,40,50,60,120,180 min);在离心法中,比较了不同离心转速(1000~12000 r/min)和离心时间(5,10,20,30 min)下的虫卵富集率,筛选出最适的离心转速和时间组合。结果表明:漂浮法富集虫卵时,选用饱和CaCl溶液漂浮30 min,可得到最高的虫卵富集率(42.1%);离心法富集虫卵时,使用10000 r/min和10 min的参数组合可得到最适的虫卵富集率(62.2%)。说明通过优化漂浮法和离心法的试验条件参数,可优化棘球绦虫卵的富集方法,从而提高虫卵富集率。

In order to establish a quick efficient method for the enrichment of Echinococcus eggs,this experiment discussed the setting of the key test conditions of flotation and centrifugation methods for the enrichment of Echinococcus eggs.In the flotation method,the enrichment rates of eggs were compared by using saturated solutions of sucrose,CaCl_(2)、NaCl、MgSO_(4)、ZnCl_(2) and ZnSO_(4) as floating solutions;and the optimal flotation durations(10,20,30,40,50,60,120,180 min)were screened out.In the centrifugation method,the enrichment rates of eggs were compared by using different centrifugation speeds(1000-12000 r/min)and centrifugation durations(5,10,20,30 min),and the optimal combination of centrifugation speed and duration was explored.The results showed that when flotation method was used for the enrichment of eggs,the saturated CaClsolution for 30 minutes was screened out,which could obtain the highest rate of the enrichment of eggs(42.1%).When centrifugation method was used for the enrichment of eggs,the parameter combination of 10000 r/min and 10 min was used,and the optimal rate of the enrichment of eggs was obtained(62.2%).The results suggested that by optimizing the test condition parameters of flotation and centrifugation methods,the enrichment method of Echinococcus eggs could be optimized for the improvement of the enrichment of eggs.