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唾液腺基底细胞腺瘤类器官模型的建立 被引量:1

Establishment of patient-derived salivary gland basal cell adenoma organoids
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摘要 目的构建人唾液腺基底细胞腺瘤(basal cell adenoma,BCA)体外类器官模型。方法纳入2021年10月于上海交通大学医学院附属第九人民医院口腔病理科确诊的唾液腺BCA患者1例(66岁女性),收集其手术切除新鲜肿瘤组织,在以基质胶固态液滴为基础的培养液中行体外类器官培养,倒置显微镜观察类器官生长情况;14 d后将类器官用4%中性多聚甲醛固定,琼脂预包埋石蜡包埋法制成石蜡块,切片、苏木精-伊红染色,组织学形态观察及p63、Ki67、细胞角蛋白14(cytokeratin14,CK14)、β-联蛋白(β-catenin)、S-100、钙调理蛋白(calponin)免疫组化染色进行类器官鉴定。结果构建的BCA类器官光镜下局部呈分叶结节状,类器官细胞巢周围嗜伊红玻璃样物质沉积,与BCA组织形态学相似;免疫组化显示类器官细胞表达CK14、p63,β-联蛋白核阳性,提示类器官保留了BCA原肿瘤细胞的免疫表型。结论初步建立了人唾液腺良性肿瘤BCA体外类器官模型。 Objective To establish an in vitro organoid model of human salivary gland basal cell adenoma(BCA).Methods Fresh tumor sample from a 66-year-old female patient diagnosed with salivary gland BCA was collected from the Dpartment of Oral pathology,Ninth People′s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine in October 2021.And the organoid culture was performed in vitro in a culture medium based on solid droplets of matrix gel,and the growth of the organoid was observed by inverted microscopy.After 14 days,the organoid was fixed in 10%neutral formalin and made into paraffin blocks by agar pre-embedding paraffin embedding method,sectioned.HE staining,morphological observation and immunohistochemical staining of p63,Ki-67,cytokeratin14(CK14),β-catenin,S-100 and calponin were used for organoids identification.Results The established BCA organoids were lobulated nodular locally under light microscopy,with deposition of eosinophilic glass-like material around the nests of organoid cells,similar to the morphological architectures of the parental BCA.Immunohistochemistry showed that organoids expressed CK14,p63,andβ-catenin in various degree,which was consistent with the immunophenotypic characteristics of the parental BCA tumor cells.Conclusions An in vitro culture system of BCA organoids was preliminarily established which provides a new model for the study of the pathogenesis of salivary gland tumors.
作者 陈宛灵 石超吉 薛俊青 张春叶 胡宇华 孙晶晶 王敏 黄新懿 田臻 Chen Wanling;Shi Chaoji;Xue Junqing;Zhang Chunye;Hu Yuhua;Sun Jingjing;Wang Min;Huang Xinyi;Tian Zhen(Department of Oral Pathology,Shanghai Ninth People′s Hospital,Shanghai Jiao Tong University School of Medicine&College of Stomatology,Shanghai Jiao Tong University&National Center for Stomatology&National Clinical Research Center for Oral Diseases&Shanghai Key Laboratory of Stomatology&Shanghai Research Institute of Stomatology,Shanghai 200011,China)
出处 《中华口腔医学杂志》 CAS CSCD 北大核心 2022年第11期1141-1146,共6页 Chinese Journal of Stomatology
基金 上海市科技计划(22ZR1437000)。
关键词 类器官 基底细胞腺瘤 涎腺 临床前模型 Organoids Basal cell adenoma Salivary glands Preclinical models
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