摘要
在基因编辑领域,以CRISPR-Cas9(clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9)为代表的技术的广泛应用,使得科学家能快速、高效、精确地对靶基因序列进行遗传改造。但是,由于包括人类在内的真核细胞二倍性的特点,要获得纯合编辑的细胞系和模式动物仍需大量的时间和人力成本。类精子干细胞(DKO-AG-haESCs)系统的建立及其与CRISPR-Cas9技术的结合则可以快速地在基因、染色体等水平上进行任意遗传操作并转化为动物个体,实现疾病模拟。本文将对类精子干细胞介导的遗传改造进行总结和讨论。
The extensive application of technology represented by CRISPR/Cas9(clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9),make the genetic modification more efficient.However,it remains a laborious endeavor to obtain homozygous gene-edited cell lines and model animals because of the diploidy of human cells and model organisms.The establishment of sperm-like stem cells(DKO-AG-haESCs)and its combination with CRISPR-Cas9 technology can rapidly transform genetic operation at the gene level and chromosome level into animals so that we can realize disease simulation.Here,we will summarize the sperm-like stem cells mediated gene editing.
作者
杨振华
李劲松
YANG Zhen-Hua;LI Jin-Song(School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China;State Key Laboratory of Cell Biology,Shanghai Key Laboratory of Molecular Andrology,Shanghai Institute of Biochemistry and Cell Biology,Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences,Shanghai 200031,China;University of Chinese Academy of Sciences,Beijing 100049,China;School of Life Science,Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Hangzhou 310024,China)
出处
《生命科学》
CSCD
北大核心
2022年第10期1227-1239,共13页
Chinese Bulletin of Life Sciences
基金
国家自然科学基金项目(31821004,32030029,31730062)。
关键词
基因编辑
类精子干细胞
疾病模拟
基因组标签计划
染色体改造
gene editing
sperm-like stem cells
disease simulation
genome tagging project
chromosome modification
