摘要
Urothelial carcinoma(UC)is a common malignant tumor in the urinary system with high recurrence rate and low survival rate 5 years after surgery.At present,imaging examination and other diagnostic methods have some shortcomings such as invasiveness and non-specificity.Therefore,it is urgent to develop a simple,rapid,noninvasive,highly sensitive and highly specific strategy to diagnose UC.Herein,a high-performance fluorescence sensor was constructed by the plasmonic gold nanorods(AuNRs)-enhanced near-infrared(NIR)fluorescence of silver sulfide quantum dots(Ag_(2)S QDs).The designed sensor can be used for the fast and accurate detection of small molecule single-transmembrane protein(FXYD3),which is overexpressed in 90%of ureteral cancers and 84%of high-grade bladder cancers.Due to its high specificity,the NIR fluorescence sensor achieves the detection of FXYD3 in the range of 0.25-150 ng·ml^(-1)with a detection limit of 0.2 ng·ml^(-1).Importantly,it also can be used for accurate diagnosis of FXYD3 in the urine of patients with relevant cancers,and the results are consistent with clinical cystoscopy and pathological analysis.The proposed fluorescence sensor provides a simple,ultrasensitive,reliable method for UC screening,tumor-grade classification and postoperative monitoring and will have great potential for clinical applications.
出处
《Rare Metals》
SCIE
EI
CAS
CSCD
2022年第11期3828-3838,共11页
稀有金属(英文版)
基金
financially supported in part by the National Natural Science Foundation of China(Nos.22005081,51873222 and 52111530128)
Zhejiang Provincial Natural Science Foundation of China(Nos.LY22B050003 and LZ22B050001)
the Funding for the Scientific Research Foundation for Scholars of Hangzhou Normal University(Nos.4095C5021920467 and 4095C5021920452)
the Key Research and Development Projects of Anhui Province(Nos.202004g01020016 and 202104g01020009)。