中国朝鲜族9个STR基因座遗传多态性研究

Genetic Polymorphism of 9 STR loci in Chaoxian National Minority of China

为丰富中华民族基因数据库,获取中国吉林省特有少数民族--朝鲜族D3S1358、vWA、FGA、TH01、TPOX、CSF1PO、D5S818、D13S317、D7S820等9个STR基因座的群体遗传数据。采用四色荧光标记STR基因扫描技术,检测91个无关个体血液样本。结果共检出81种等位基因,其基因频率分布在0.0055～0.4615之间;共检出196种基因型,其基因型频率分布在0.0110～0.9890之间。9个STR基因座基因型频率观察值与期望值均符合Hardy-Weinberg平衡定律(P>0.05)。9个基因座的多态信息量PIC(polymorphicinformationcontent)分布于0.6863～0.8807之间,杂合度H(heterozygosity)分布于0.6919～0.8809之间,个体识别力DP(discriminationpower)分布于0.8301～0.9670之间,非父排除率PPE(probabilityofpaternityexclusion)分布于0.8590～0.9942之间。研究结果可应用于人类群体遗传学及法医学研究等领域。

In order to enrich the Chinese genetic database,nine polymorphic loci of STR,such as D3S1358,vWA,FGA,TH01,TPOX,CSF1PO,D5S818,D13S317 and D7S820 were studied.Based on STR gene scan marked by fluorescence,91 unrelated Chinese Chaoxian individuals were observed.81 alleles and 196 genotypes were found.The corresponding gene frequency and genotype frequency were 0.0055～0.4615 and 0.0110～0.9890 respectively.The genogypes frequency of nine STR loci was good with the Hardy-Weinberg equilibrium(P>0.05).The statistical analysis of nine STR loci showed the following:PIC(polymorphic information content)≥0.6863,H(heterozygosity)≥0.6919,DP(discrimination power)≥0.8301,EPP(probability of paternity exclusion)≥0.8590.The data studied can be used in Chinese population genetic studies and forensic medicine applications.