胶质瘤中hsa_circ_0021350的表达情况及临床意义研究

A study on the expression of hsa_circ_0021350 in glioma and its clinical significance

目的探讨hsa_circ_0021350在胶质瘤中的表达及临床意义,初步评价其应用于胶质瘤诊断和治疗的潜力。方法选择2016年1月至2022年5月在广西医科大学第一附属医院神经外科经手术获取的胶质瘤组织标本40例(高级别胶质瘤22例,低级别胶质瘤18例),正常脑组织标本10例。通过RNase R消化联合逆转录聚合酶链反应(RT-PCR)验证hsa_circ_0021350的环状特性。通过TA克隆测序法鉴定实时荧光定量PCR(qRT-PCR)扩增产物。比较高级别胶质瘤、低级别胶质瘤和正常脑组织hsa_circ_0021350的表达水平。分析hsa_circ_0021350表达与胶质瘤患者临床病理指标的关联性。结果该研究所设计的发散引物可有效特异扩增hsa_circ_0021350。经验证,hsa_circ_0021350呈环状,且能耐受RNase R消化。qRT-PCR实验结果显示,高级别胶质瘤和低级别胶质瘤hsa_circ_0021350的表达水平均高于正常脑组织,且高级别胶质瘤hsa_circ_0021350的表达水平较低级别胶质瘤更高,差异有统计学意义(P<0.05)。hsa_circ_0021350的表达与胶质瘤分级和ki67阳性率具有显著关联性(P<0.05)。结论hsa_circ_0021350在胶质瘤,尤其是高级别胶质瘤中呈高表达,且与肿瘤增殖因子ki67相关,是胶质瘤诊断和治疗的潜在分子靶点。

Objective To investigate the expression of hsa_circ_0021350 in glioma and its clinical significance,and to preliminarily evaluate its potential application in the diagnosis and treatment of glioma.Methods Forty glioma tissue specimens(including 22 cases of high-grade gliomas and 18 cases of low-grade gliomas)and 10 normal brain tissue specimens were collected in Department of Neurosurgery,the First Affiliated Hospital of Guangxi Medical University,from January 2016 to May 2022.The ring-like properties of hsa_circ_0021350 were verified by RNase R digestion combined with reverse transcription-polymerase chain reaction(RT-PCR).The amplification products of quantitative real-time PCR(qRT-PCR)were identified by TA clone sequencing.The expression levels of hsa_circ_0021350 were compared among the high-grade gliomas,the low-grade gliomas and the normal brain tissues.The correlation between hsa_circ_0021350 expression and clinicopathological indicators was analyzed in the glioma patients.Results The divergent primers designed in this study could effectively and specifically amplify hsa_circ_0021350,and hsa_circ_0021350 was ring-shaped and tolerant to RNase R digestion.The experimental results of qRT-PCR showed that the expression levels of hsa_circ_0021350 in the high-grade gliomas and the low-grade gliomas were higher than those in the normal brain tissues,and the expression level of hsa_circ_0021350 in the high-grade gliomas was higher than that in the low-grade gliomas,and the differences were statistically significant(P<0.05).The expression of hsa_circ_0021350 was significantly correlated with the grade of glioma and the positive rate of ki67(P<0.05).Conclusion Hsa_circ_0021350 is highly expressed in gliomas,especially in high-grade gliomas,and is related to tumor proliferation factor ki67.hsa_circ_0021350 can be used as a potential molecular target for diagnosis and treatment of gliomas.