水胡桃叶中萘醌的提取及其体外抗氧化活性

Optimization of Extraction Process and in Vitro Antioxidant Activity of Naphthoquinones from Leaves of Pterocarya rhoifolia Sieb. et Zucc

为探讨水胡桃叶提取物体外抗氧化活性,试验以乙醇为溶剂,从水胡桃中提取萘醌。在单因素试验基础上,通过正交试验对萘醌的提取条件进行优化;测定提取物还原力,以及对DPPH·和·OH自由基的清除率。结果表明,从水胡桃叶中提取萘醌的最优提取条件:料液比1︰50 (g/mL),提取时间5 h,提取温度60℃,乙醇体积分数50%。萘醌的提取率可达168.14 mg/g。萘醌提取物可以较好地清除DPPH·自由基和·OH自由基,具有较强的还原力,体现一定的体外抗氧化活性。试验结果对天然植物中抗氧化剂的开发利用具有重要指导意义。

In order to study the antioxidant activity of walnut leaf extract in vitro, naphthoquinone was extracted from walnut leaf with ethanol as solvent. On the basis of single factor test, orthogonal test was used to optimize the extraction conditions of naphthoquinone, and to determine the reducing power of the extract, the radical scavenging rate DPPH· and ·OH. The results showed that the optimal extraction conditions were as follows: solid-liquid ratio of 1︰50(g/mL), extraction time of 5h, extraction temperature of 60 ℃ and ethanol concentration of 50%. Under this condition, extraction yield of naphthoquinone could reach 168.14 mg/g. Naphthoquinone extract could effectively scavenge DPPH· and ·OH free radical, and had strong reducing power, showing good antioxidant activity in vitro. The results had important significance for the development and utilization of antioxidants in natural plants.