HPLC法同时测定双葛止泻口服液中绿原酸和葛根素的含量

Determination of Chlorogenic Acid and Puerarin in Shuangge Zhixie Oral Liquid by HPLC

建立了同时测定双葛止泻口服液中绿原酸和葛根素含量的测定方法。采用高效液相色谱(HPLC)法,使用Waters XBridge C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-0.4%磷酸溶液梯度洗脱;检测波长为315 nm;流速为1.0 mL/min;柱温为30℃。结果表明,绿原酸浓度在0.008242~0.1030 mg/mL的范围内,与峰面积线性关系良好(r=1.0000),平均回收率为99.1%(n=6),RSD为1.8%;葛根素浓度在0.02296~0.2871 mg/mL的范围内,与峰面积线性关系良好(r=1.0000),平均回收率为98.7%(n=6),RSD为1.6%。该方法简单、准确、专属性强,可用于双葛止泻口服液中绿原酸和葛根素的质量控制。

The method for determination of the content of chlorogenic acid and puerarin in Shuangge Zhixie Oral Liquid was developed.The analysis was performed on an Waters XBridge C18 column(250 mm×4.6 mm,5μm).The gradient mobile phase was consisted of acetonitrile-0.4%phosphoric acid solution,and the detection wavelength was 315 nm.The flow rate was 1.0 mL/min and the column temperature was 30℃.The linear range of the calibration curves for chlorogenic acid was 0.008242~0.1030 mg/mL(r=1.0000),and the average recovery was 99.1%(n=6)with RSD of 1.8%.The linear range of the calibration curves for puerarin was 0.02296~0.2871 mg/mL(r=1.0000),and the average recovery was 98.7%(n=6)with RSD of 1.6%.The method is simple and accurate with high specificity,which can be used to determine the content of chlorogenic acid and puerarin in Shuangge Zhixie Oral Liquid.