摘要
建立了同时测定双葛止泻口服液中绿原酸和葛根素含量的测定方法。采用高效液相色谱(HPLC)法,使用Waters XBridge C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-0.4%磷酸溶液梯度洗脱;检测波长为315 nm;流速为1.0 mL/min;柱温为30℃。结果表明,绿原酸浓度在0.008242~0.1030 mg/mL的范围内,与峰面积线性关系良好(r=1.0000),平均回收率为99.1%(n=6),RSD为1.8%;葛根素浓度在0.02296~0.2871 mg/mL的范围内,与峰面积线性关系良好(r=1.0000),平均回收率为98.7%(n=6),RSD为1.6%。该方法简单、准确、专属性强,可用于双葛止泻口服液中绿原酸和葛根素的质量控制。
The method for determination of the content of chlorogenic acid and puerarin in Shuangge Zhixie Oral Liquid was developed.The analysis was performed on an Waters XBridge C18 column(250 mm×4.6 mm,5μm).The gradient mobile phase was consisted of acetonitrile-0.4%phosphoric acid solution,and the detection wavelength was 315 nm.The flow rate was 1.0 mL/min and the column temperature was 30℃.The linear range of the calibration curves for chlorogenic acid was 0.008242~0.1030 mg/mL(r=1.0000),and the average recovery was 99.1%(n=6)with RSD of 1.8%.The linear range of the calibration curves for puerarin was 0.02296~0.2871 mg/mL(r=1.0000),and the average recovery was 98.7%(n=6)with RSD of 1.6%.The method is simple and accurate with high specificity,which can be used to determine the content of chlorogenic acid and puerarin in Shuangge Zhixie Oral Liquid.
作者
周冰
李焕娟
陈帅韬
安卓睿
程婵婵
姚路路
张聪
ZHOU Bing;LI Huan-juan;CHEN Shuai-tao;AN Zhuo-rui;CHENG Chan-chan;YAO Lu-lu;ZHANG Cong(National Research Center for Veterinary Medicine/Luoyang Huizhong Veterinary Medicine Co.,Ltd,Luoyang 471000,China)
出处
《中国兽药杂志》
2022年第10期41-47,共7页
Chinese Journal of Veterinary Drug
基金
2019年度洛阳市科技重大专项项目(1901024A)。