右美托咪定对糖尿病神经痛大鼠疼痛水平、炎症反应及免疫功能影响的实验研究

Effects of dexmedetomidine on pain level,inflammatory response and immune function in diabetic neuralgia rats

目的:探讨右美托咪定对糖尿病神经痛大鼠疼痛水平、炎症反应及免疫功能的影响。方法:健康SPF级成年雄性SD大鼠32只,分为空白对照组(NC组),右美托咪定组(NC+DD组)、糖尿病神经痛组(DNP组)和糖尿病神经痛+右美托咪定组(DNP+DD组),每组8只。采用腹腔注射链脲佐菌素构建糖尿病神经痛大鼠模型。采用热辐射刺激、冷板试验、触觉过敏试验测定大鼠行为学特征;采用Von Frey电子测痛仪进行疼痛阈值测定;检测大鼠T淋巴细胞亚群及炎症因子水平。结果:与NC组相比,NC+DD组爪子对冷刺激的消退潜伏期、爪子对热刺激的消退潜伏期、爪子机械刺激退缩反应明显更低(均P<0.05),DNP组和DNP+DD组冷刺激的消退潜伏期、爪子对热刺激的消退潜伏期、爪子机械刺激退缩反应明显高于NC组和NC+DD组(均P<0.05),且DNP组显著高于DNP+DD组(P<0.05)。1、7 d和14 d与NC组比较,NC+DD组机械撤退阈值明显更高(均P<0.05),DNP组和DNP+DD组机械撤退阈值明显低于NC组和NC+DD组(均P<0.05),且DNP组显著低于DNP+DD组(P<0.05)。与NC组相比,NC+DD组CD3^(+)、CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+)明显更高(均P<0.05),DNP组和DNP+DD组CD3^(+)、CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+)明显低于NC组和NC+DD组(均P<0.05),且DNP组显著低于DNP+DD组(P<0.05)。与NC组相比,NC+DD组TNF-α、IL-1β、IL-18明显更低(均P<0.05),DNP组和DNP+DD组TNF-α、IL-1β、IL-18明显高于NC组和NC+DD组(均P<0.05),且DNP组显著高于DNP+DD组(P<0.05)。结论:糖尿病神经痛大鼠采用右美托咪定,可调节T细胞免疫功能,抑制炎症因子的表达,进而提高大鼠疼痛阈值改善疼痛行为表现。

Objective:To investigate the effects of dexmedetomidine on pain level,inflammatory response and immune function in diabetic neuralgia rats.Methods:Thirty-two healthy SPF adult male SD rats were divided into blank control group(NC group),dexmedetomidine group(NC+DD group),diabetic neuralgia group(DNP group)and diabetic neuralgia+dexmedetomidine group(DNP+DD group),with 8 rats in each group.The rat model of diabetic neuralgia was established by intraperitoneal injection of streptozotocin.The behavioral characteristics of rats were measured by thermal radiation stimulation,cold plate test and tactile hypersensitivity test.The pain threshold was measured by Von Fery thin wire tactile pain meter.The levels of T lymphocyte subsets and inflammatory factors were detected.Results:Compared with the NC group,the regression latency of paws to cold stimulation,the regression latency of paws to heat stimulation,and the withdrawal response of paws to mechanical stimulation were significantly lower in the NC+DD group(all P<0.05).The regression latency of cold stimulation,regression latency of paws to thermal stimulation,and withdrawal response of paws to mechanical stimulation in DNP group and DNP+DD group were significantly higher than those in NC group and NC+DD group(all P<0.05),and DNP group was significantly higher than DNP+DD group(P<0.05).On day 1,7 and 14,the mechanical withdrawal threshold of NC+DD group was significantly higher than that of NC group,and the mechanical withdrawal threshold of DNP group and DNP+DD group was significantly lower than that of NC group and NC+DD group,and the mechanical withdrawal threshold of DNP group was significantly lower than that of DNP+DD group(all P<0.05).Compared with NC group,CD3^(+),CD4^(+),CD8^(+)and CD4^(+)/CD8^(+)in NC+DD group were significantly higher,and CD3^(+),CD4^(+),CD8^(+)and CD4^(+)/CD8^(+)in DNP and DNP+DD groups were significantly lower than those in NC and NC+DD groups,and DNP group was significantly lower than DNP+DD group(all P<0.05).TNF-α,IL-1βand IL-18 in NC+DD group were significantly lower than those in NC group,and TNF-α,IL-1βand IL-18 in DNP and DNP+DD groups were significantly higher than those in NC group and NC+DD group,and DNP group was significantly higher than DNP+DD group(all P<0.05).Conclusion:Dexmedetomidine in rats with diabetic neuralgia can regulate the immune function of T cells,inhibit the expression of inflammatory factors,and then increase the pain threshold of rats to improve pain behavior.