改善口臭的乳杆菌筛选及口腔益生特性评价

Screening and evaluation of Lactobacillus for improving halitosis

筛选到能够有效改善口臭并且具有良好口腔益生特性的口腔益生菌。通过抑制H_(2)S和挥发性硫化物的产生、降低产硫化物基因的表达量、抑制生物膜形成量等指标对乳杆菌进行筛选评估。评价2株有效的乳杆菌(植物乳杆菌CCFM1214和唾液乳杆菌CCFM1215)产H_(2)O_(2)的能力、自聚能力以及与具核梭杆菌的共聚能力。结果表明,植物乳杆菌CCFM1214和唾液乳杆菌CCFM1215均能显著降低具核梭杆菌的挥发性硫化物(volatile sulfur compounds,VSCs)的产生量、生物膜形成量及生长,效果显著优于唾液链球菌K12,并且有良好的自聚、共聚的能力。经过口腔益生特性评价,植物乳杆菌CCFM1214和唾液乳杆菌CCFM1215均有作为改善口臭的口腔益生菌的应用潜力。

Halitosis,also known as bad breath or oral malodor,refers to the occurrence of foul-smelling gases when breathing.Halitosis is not only an oral disease,but also has negative impact on patients′social communication and mental health.Halitosis is caused by the decomposition of sulfur-containing amino acids or serum by oral anaerobic microorganisms,resulting in metabolites dominated by volatile sulfur compounds(VSCs),especially H_(2)S.Fusobacterium nucleatum is one of the main H 2S producing strain.Probiotics can inhibit the growth and biofilm formation of pathogens,regulate oral flora,and it is reported to be good for oral health.The aim of this study was to screen oral probiotics which can effectively improve halitosis from 148 Lactobacillus strains isolated from difference sources.Among them,28 strains had the ability to inhibit F.nucleatum producing H_(2)S.Then the 28 Lactobacillus strains were evaluated by the effects on the VSCs producing,the Fn1220 gene expression and the biofilm formation ability of F.nucleatum.The commercial probiotic Streptococcus saliva K12 was set as a control.Two strains,Lactiplantibacillus plantarum CCFM1214 and Ligilactobacillus salivarius CCFM1215 were selected due to their significant inhibition rate of 90.94%and 90.19%,respectively.At the same time,the inhibition effect of S.saliva K12 was 71.16%.The supernatant of L.plantarum CCFM1214 and L.salivarius CCFM1215 could also inhibit the expression of Fn1220 gene and the formation of F.nucleatum biofilm,which were significantly better than S.saliva K12.As a result,L.plantarum CCFM1214 and L.salivarius CCFM1215 could both inhibit the growth of F.nucleatum.Besides,they also had good self-aggregation,co-aggregation ability and H_(2)O_(2)production ability.After the evaluation of oral probiotics characteristics,they had the application potential as oral probiotics to improve halitosis.These results contribute to the application of probiotics in the field of oral cavity and the development of the diversity of probiotic strains in functional food.