不同产地及种类桑枝中25种元素的测定与安全性评价

Determination and safety evaluation of 25 elements in mulberry branches of different varieties from different origin

建立了采用凯氏定氮法、电感耦合等离子体质谱法和原子荧光光谱法测定不同产地及种类桑枝中N、Ca、K、P、Mg、Al、Fe、Zn、Cu、Sr、Ba、Mn、Na、B、Pb、Ni、Cr、Mo、Sn、Co、As、Se、Cd、Sb、Hg等25种元素的方法,并对测定结果进行了分析,以评价其质量与安全性。结果表明,除N以外的24种元素在其各自浓度范围内呈现良好的线性关系,相关系数R^(2)≥0.9992;18个批次的桑枝均检出了25种元素,但各批次含量组成差异较大;25种元素中平均含量最高的元素为Ca(13.23 mg/g),其次为N(12.75 mg/g)、K(7.99 mg/g)、P(2.62 mg/g)、Mg(1.39 mg/g),平均含量最低的元素为Hg(0.022μg/g);所有批次桑枝中Cu及重庆市巴南区安澜镇的鸡桑(S4)中的Pb超出标准限量值;主成分分析表明,重庆市巴南区安澜镇的鸡桑(S4)主成分综合得分最高,其次为重庆市南岸区黄桷垭镇的鸡桑(S8)、重庆市南岸区黄桷垭镇的华桑(S9)和重庆市奉节县新民镇的鸡桑(S14);聚类分析结果显示,重庆市巴南区安澜镇的鸡桑(S4)为一类,重庆市南岸区黄桷垭镇的鸡桑(S8)、重庆市南岸区黄桷垭镇的华桑(S9)和重庆市奉节县新民镇的鸡桑(S14)为一类,其余14批桑枝样品为一类。该研究可为桑枝的质量与安全性评价以及药用价值的开发利用提供理论依据和基础资料。

The contents of 25 elements such as N,Ca,K,P,Mg,Al,Fe,Zn,Cu,Sr,Ba,Mn,Na,B,Pb,Ni,Cr,Mo,Co,As,Se,CD,Sb,and Hg in 18 mulberry branch samples from different producing areas were determined by automatic Kjeldahl nitrogen determination method,inductively coupled plasma mass spectrometry and atomic fluorescence spectrometry to evaluate the quality and safety of mulberry branches.Result showed that 24 elements expressed a good linear relationship in their respective concentration ranges except for N,and with a correlation coefficient that R^(2)≥0.9992.The 25 elements were detected in all of the 18 mulberry branch samples and there is a large difference in the content structure of the 25 elements between samples.The highest average content was Ca(13.23 mg/g),followed by N(12.75 mg/g),K(7.99 mg/g),P(2.62 mg/g),Mg(1.39 mg/g),and the lowest average content was Hg(0.022μg/g).The Pb content in S4(Morus australis from Anlan town,Ba’nan District,Chongqing)and the Cu contents in all samples exceeded the standard limits.The sample S4 had the highest general score of principal components,followed by S8(M.australis from Huangjueya Town,Nan′an District,Chongqing),S9(M.cathayana from Huangjueya Town,Nan′an District,Chongqing),and S14(M.australis from Xinmin Town,Fengjie county,Chongqing).The result of cluster analysis showed that S4 could be clustered as the first group,S8,S9,and S14 could be clustered as the second group,and the rest 14 samples could be clustered in the third group.This study could provide theoretical and basic data for the quality and safety evaluation,and the development and utilization of the medicinal value of the mulberry branch.