巴特日-7对小鼠放射性肠损伤的防治作用研究

Preventive and therapeutic effects of Bateri-7 on radiation-induced intestinal injury in mice

目的:探讨蒙药巴特日-7对12 Gy 60Coγ射线全身照射小鼠肠道辐射损伤的防护作用。方法:C57BL/6J雄性小鼠按随机数表法分为对照组、照射组和照射给药组,每组15只。存活实验分为照射组和照射给药组,每组10只。12 Gy 60Coγ射线全身单次照射,对照组和照射组给予生理盐水灌胃,照射给药组给予巴特日-7(530 mg/kg)灌胃,给药方式为照射前7 d和照射后3 d连续给药。照射后6和24 h免疫组织化学法检测Tunel阳性细胞;照射后3.5 d HE染色观察肠道绒毛结构,免疫组织化学法检测BrdU、Ki67阳性细胞;实时荧光定量PCR(qPCR)检测小肠白介素6(IL-6)、肿瘤坏死因子α(TNF-α)和趋化因子5(Cxcl-5)表达水平;测定外周血异硫氰酸荧光素-葡聚糖(FITC-dextran)含量。结果:照射给药组小鼠存活时间长于照射组(χ2=5.84,P<0.05),小鼠体重变化两组间差异无统计学意义(P>0.05);照射给药组小肠绒毛长度明显大于照射组(t=20.24,P<0.05),两组小肠隐窝深度比较差异无统计学意义(P>0.05)。照射后6和24 h,照射给药组肠隐窝Tunel阳性细胞数量较照射组明显减少(t=3.52、2.90,P<0.05)。照射后3.5 d,照射给药组小鼠血清FITC-dextran水平、小肠组织IL-6、TNF-α和Cxcl-5的表达均明显低于照射组(t=6.92、7.01、7.18、13.16,P<0.05);而BrdU、Ki67阳性隐窝数高于照射组(t=3.91、2.57,P<0.05)。结论:蒙药巴特日-7能够有效改善小鼠辐射后肠道损伤,对放射性肠炎具有较好的防治作用,为药物治疗放射性肠炎提供新的依据。

Objective To study the protective effect of Mongolian medicine Bateri-7 on radiation-induced intestinal injury in mice.Methods C57BL/6J male mice were randomly divided into control group,irradiation group and irradiation plus drug administration group,with 10 or 15 mice in each group.For irradiation group,the mice were given a single dose of 12 Gy 60Coγ-rays with total body irradiation.For drug treatment,the mice were gavaged with Bateri-7(530 mg/kg)7 d before irradiation until 3 d after IR.At 6 h and 24 h after irradiation,the Tunel positive cells in intestine were detected immunohistochemically.At 3.5 d after irradiation,the structure of intestinal villi was observed by HE staining,and the BrdU and Ki67 positive cells were detected immunohistochemically.The expression levels of IL-6,TNF-αand Cxcl-5 were detected by qPCR.The FITC-dextran in peripheral blood was also determined.Results The survival of irradiated mice was significantly increased by Bateri-7(χ2=5.84,P<0.05),but there was no significant difference in weight between two groups(P>0.05).The villi length of small intestine in the irradiation plus drug group was significantly longer than that in the irradiation group(t=20.24,P<0.05),and there was no significant difference in the depth of intestinal crypt between two groups(P>0.05).At 6 and 24 h after irradiation,the number of Tunel positive cells in intestinal crypts in the irradiation plus drug group was significantly reduced in comparison with the irradiation group(t=3.52,2.90,P<0.05).At 3.5 d after irradiation,the level of FITC-dextran in serum and the expressions of IL-6,TNF-αand Cxcl-5 in small intestine of mice in the irradiation plus drug group were significantly lower than those in the irradiation group,respectively(t=6.92,7.01,7.18,13.16,P<0.05).The number of BrdU and Ki67 positive cells in the crypt of mice in the irradiation plus drug group was higher than that of the irradiation group(t=3.91,2.57,P<0.05).Conclusions Mongolian medicine Bateri-7 can effectively alleviate irradiation-induced intestinal injury of mice,which may have a good preventive and therapeutic effect on radiation enteritis.