布莱克韦尔虫草的生物活性评价及其子实体的人工培育

Evaluation of biological activities and artificial cultivation of fruiting bodies of Cordyceps blackwelliae

虫草在世界范围内广泛分布,具有多种生物活性及较高的食药用价值。为进一步丰富虫草真菌资源,本研究对从广东韶关丹霞山采集到的一份野生虫草样品进行了菌株的分离鉴定、生物活性评价和子实体的诱导培养。通过形态学和系统发育分析,将菌株ZYJ0835鉴定为布莱克韦尔虫草Cordyceps blackwelliae。利用5种不同的培养基进行液体发酵培养,发现该菌株的发酵上清液和菌丝体均具有抗氧化和纤溶活性。此外,该菌株还具有一定的抑菌活性。利用大米、小麦、柞蚕蛹3种培养基质,均成功诱导出子实体,并且人工培养的子实体也具有抗氧化和纤溶活性。本研究首次在中国境内报道布莱克韦尔虫草的分布,为进一步开发利用该虫草资源提供了理论依据。

Cordyceps fungi are widely distributed throughout the world,with a variety of biological activities and great potential for use as food and medicine.In order to enrich and develop natural resources of cordyceps fungi,a wild Cordyceps fruiting body was sampled from Danxia Mountain,Shaoguan,Guangdong,China.The fungal isolate ZYJ0835 obtained from the natural fruiting body was employed to evaluate its biological activities and probability of artificial cultivation.Based on morphology and multi-gene phylogenetic analyses,ZYJ0835 was identified as Cordyceps blackwelliae.Five different media were used for liquid fermentation,and it was found that fermentation supernatant and mycelia of the strain showed both antioxidant and fibrinolytic activities.In addition,the strain also showed antimicrobial activities to some extent.Fruiting bodies were successfully induced on solid media consisted of rice,wheat,or tussah pupae,and the artificially-cultivated fruiting bodies also displayed antioxidant and fibrinolytic activities.This study reports,for the first time,the distribution of C.blackwelliae in China,and development and utilization of this cordyceps resource can be expected.