TYMS蛋白在原发性肝癌中的表达及预后评估价值分析

Expression of TYMS protein in primary liver cancer and its prognostic evaluation value analysis

目的探讨胸苷酸合成酶(TYMS)蛋白在原发性肝细胞癌(HCC)中的表达水平及其对临床预后的评估价值。方法采用免疫组织化学染色法检测100例经病理学诊断为HCC患者癌组织及癌旁组织TYMS蛋白的表达情况,并进行量化评分,分析TYMS蛋白在不同临床病理特征患者中的表达情况,以及TYMS蛋白表达水平与HCC患者生存率的关系。结果TYMS蛋白表达水平在不同性别、年龄、肿瘤最大径、甲胎蛋白水平、乙型肝炎病毒-DNA水平等患者间比较,差异无统计学意义(P>0.05),低分化者TYMS蛋白表达阳性率(83.87%)高于中-高分化者(62.32%),差异有统计学意义(P<0.05)。TYMS蛋白在HCC癌组织的表达水平高于癌旁组织,差异有统计学意义(P<0.01)。3年总生存期分析结果显示,TYMS蛋白低表达者生存率(61.3%)明显高于高表达者(22.6%),差异有统计学意义(P<0.05)。结论TYMS蛋白在HCC癌组织中表达明显异常且与肿瘤组织分化程度、患者预后密切相关。抑制TYMS蛋白表达对HCC患者的治疗和预后有重要意义。

Objective To investigate the expression level of thymidylate synthase(TYMS)protein in primary hepatocellular carcinoma(HCC)and its clinical prognostic evaluation value.Methods The immunohistochemistry staining was used to detect the expression of TYMS protein in liver cancer tissues and paracancerous tissues of 100 patients with pathologically diagnosed HCC and the quantitative scoring was performed;the expression situation of TYMS protein in tissues with different clinicopathological features patients and the relationship between TYMS protein expression level and survival rate of the patients with HCC were analyzed.Results There was no statistically significant difference in the expression level of TYMS protein among the patients with different genders,ages,tumor maximal diameter,AFP level,HBV-DNA level etc.(P>0.05),the positive rate of TYMS protein expression in the low differentiation patients was 83.87%,which was higher than 62.32%in the medium differentiation and high differentiation patients,and the differences were statistically significant(P<0.05).The expression level of TYMS protein in HCC cancer tissues was higher than that in paracancerous tissues,and the difference was statistically significant(P<0.01),The 3-year overall survival analysis showed that the survival rate of the patients with TYMS protein low expression was 61.3%,which was significantly higher than 22.6%of the patients with TYMS protein high expression,and the difference was statistically significant(P<0.05).Conclusion The TYMS protein expression is significantly abnormal in HCC cancer tissue and closely correlated to the differentiation degree of cancer and the prognosis of the patients.Inhibiting TYMS protein expression has an important significance for the treatment and prognosis of the patients with HCC.