ADAM33基因T2位点多态性与支气管哮喘易感性的关联研究

Study of association between T2 locus polymorphism of ADAM33 gene and susceptibility to bronchial asthma

目的研究解整合素-金属蛋白酶33(ADAM33)基因T2位点多态性与支气管哮喘易感性的关联性。方法选取2019年12月至2020年12月在该院就诊并接受治疗的支气管哮喘患者120例作为研究组,选择同期在该院体检的健康者120例作为对照组,采用等位基因特异性聚合酶链反应技术及DNA测序方法检测两组ADAM33基因T2位点多态性。结果研究组和对照组ADAM33基因T2位点3种基因型均符合Hardy-Weinberg平衡定律(P>0.05)。研究组与对照组ADAM33基因T2位点基因型(AA、AG、GG)频率比较,差异均有统计学意义(χ^(2)=7.001,P<0.05)。ADAM33基因T2位点等位基因中,研究组A等位基因频率(15.42%)低于对照组(23.75%),差异有统计学意义(P<0.05)。ADAM33基因T2位点A等位基因频率增加可降低支气管哮喘患病风险(OR=0.875,P<0.05)。与中度支气管哮喘患者相比,危重、重度支气管哮喘患者ADAM33基因T2位点AG基因型频率差异无统计学意义(P>0.05),中度、重度、危重支气管哮喘患者ADAM33基因T2位点AG基因型频率高于轻度支气管哮喘患者,差异均有统计学意义(P<0.05)。ADAM33基因T2位点AG基因型频率增加是影响支气管哮喘严重程度的高危因素(P<0.05)。结论支气管哮喘与ADAM33基因位点多态性存在关联:ADAM33基因T2位点与支气管哮喘易感性有关,ADAM33基因T2位点A等位基因频率增加可降低支气管哮喘的患病风险,ADAM33基因T2位点AG基因型频率增加是影响支气管哮喘严重程度的高危因素。

Objective To study the association between the T2 locus polymorphism of A distintegrin and metalloproteinase 33(ADAM33)gene and the susceptibility to bronchial asthma.Methods One hundred and twenty patients with bronchial asthma treated in this hospital from December 2019 to December 2020 were selected as the study group,and 120 healthy people undergoing physical examination in this hospital during the same period were selected as the control group.The allele specific polymerase chain reaction and DNA sequencing were used to detect the T2 locus polymorphism of ADAM33 gene in the two groups.Results The three genotypes of ADAM33 gene T2 locus in the study group and control group conformed to the Hardy-Weinberg equilibrium law(P>0.05).The frequencies of ADAM33 gene T2 locus genotypes(AA,Ag,GG)had statistical difference between the study group and control group(χ^(2)=7.001,P<0.05).Among the alleles at T2 locus of ADAM33 gene,the frequency of A allele in the study group(15.42%)was lower than that in the control group(23.75%),and the differences were statistically significant(P<0.05).The decreasing of A allele in the T2 locus of ADAM33 gene could increase the risk of suffering from bronchial asthma(OR=0.875,P<0.05).Compared with the patients with moderate bronchial asthma,the AG genotype frequency at T2 locus of ADAM33 gene in the patients with critical,severe bronchial asthma had no statistical difference(P>0.05).The frequency of AG genotype frequency at T2 locus of ADAM33 gene in the patients with moderate,severe and critical bronchial asthma was higher than that in the patients with mild bronchial asthma,and the differences were statistically significant(P<0.05).The AG genotype frequency increase at T2 locus of ADAM33 gene was a high risk factor affecting the severity of bronchial asthma(P<0.05).Conclusion Bronchial asthma has the correlation with the ADAM33 gene locus polymorphism.The T2 locus of ADAM33 gene is associated with the susceptibility to bronchial asthma.The A allele frequency increase at T2 locus of ADAM33 gene could decrease the risk of bronchial asthma.The AG genotype frequency increase at T2 locus of ADAM33 gene is a high risk factor affecting the severity of bronchial asthma.