蛋白核小球藻粉和雨生红球藻粉对中华绒螯蟹幼蟹生长和生理生化指标的影响

Effect of Chlorella pyrenoidos powder and Haematococcus pluvialis powder on growth,physiology and biochemistry index of Eriocheir sinensis

试验旨在探究饵料中添加蛋白核小球藻和雨生红球藻粉对中华绒螯蟹幼蟹生长性能、常规体成分及生理生化指标的影响。选取108只体重(7.56±0.03) g的中华绒螯蟹幼蟹随机分为3组,每组3个重复,每个重复12只。T1组中华绒螯蟹幼蟹饲喂基础饵料,T2组中华绒螯蟹幼蟹饲喂基础饵料+15%蛋白核小球藻粉,T3组中华绒螯蟹幼蟹饲喂基础饵料+15%的雨生红球藻粉。试验期42 d。结果显示:与T1组相比,T2和T3组中华绒螯蟹的增重率和特定生长率显著提高(P<0.05),T2组中华绒螯蟹超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、酸性磷酸酶(ACP)和碱性磷酸酶(AKP)活性分别提高了36.00%、34.82%、35.63%和87.30%(P<0.05),T3组中华绒螯蟹GSH-Px和AKP活性提高了39.41%和80.42%(P<0.05)。与T1组相比,T2和T3组蟹体粗蛋白含量显著提高19.86%和23.35%(P<0.05),但T2组蟹体粗脂肪含量降低14.26%(P<0.05)。氨基酸含量排序为T2>T3>T1,T2和T3组蟹体必需氨基酸/氨基酸总量分别为41.31%和41.43%,均显著高于T1组(P<0.05)。研究表明,基础饵料中添加蛋白核小球藻粉和雨生红球藻粉可以促进中华绒螯蟹幼蟹生长,改善机体抗氧化功能,优化常规体营养成分。综合考虑各项指标,饵料中添加蛋白核小球藻粉效果较好。

The experiment was to investigate the effect of feeding Chlorella pyrenoidos and Haematococcus pluvialis on growth performance, routine body composition and physiological and biochemical parameters of juvenile Eriocheir sinensis. A total of 108 juvenile eriocheir sinensis with the body weight of(7.56±0.03) g were randomly divided into three groups, with three replicates in each group and 12 crabs in each replicate. Crabs in T1 group were fed with basal diet,and crabs in T2 group and T3 group were fed with basal diet + 15% C. pyrenoidos and H. pluvialis, respectively. The experiment lasted for 42 d. The results showed that compared with T1 group, the weight gain rate and specific growth rate of crab in T2 group and T3 group were significantly increased(P<0.05). The SOD, GSH-Px, ACP and AKP in T2 group were 36.00%, 34.82%, 35.63% and 87.30% higher than those of T1 group(P<0.05), respectively. GSH-Px and AKP in T3group were increased by 39.41% and 80.42% compared with T1 group(P<0.05). The crude protein in T2 group and T3group were increased by 19.86% and 23.35% than those in control group(P<0.05), but the crude fat in T2 group was decreased by 14.26%(P<0.05). In terms of amino acids content, the modes were as T2 >T3> T1, and the EAA/TAA in T2group and T3 group were 41.31% and 41.43% higher than those of T1 group(P<0.05). The study indicates that supplementation of C. pyrenoidos and H. pluvialis in diet can improve the growth indexes, antioxidant capacity and body composition of Eriocheir sinensis. Considering all indicators together, the effect of C. pyrenoidos is better.