摘要
植物通讯信号分子β-罗勒烯在植物防御方面起着重要作用,受β-罗勒烯诱导快速高表达的SFIBO(snoRNAs Fast-Induced by Ocimene)基因的启动子特性有待研究。SFIBO基因起始密码子上游有877 bp,依据这段序列上已知的元件分布特征,将877 bp分成三段:-877~-1 bp、-637~-1 bp和-277~-1 bp。将这3个片段分别扩增后与GUS报告基因构建融合表达框,并利用浸花法将这3个植物双元表达载体转化拟南芥Col-0。对GUS组织化学染色进行分析,结果显示SFIBO基因的有效启动子为起始密码子上游637 bp DNA片段,β-罗勒烯诱导反应的顺式作用元件在-637~-277 bp之间的这360 bp DNA序列上。以上结果为下一步确定β-罗勒烯响应元件的具体序列和研究SFIBO基因的功能提供了科学依据。
Plant communication signal moleculeβ-ocimene plays an important role in plant defense.The promoter characteristics of SFIBO(snoRNAs fast induced by ocimene)gene induced byβ-ocimene need to be studied.There are 877 bp upstream of the start codon of SFIBO gene.According to the distribution characteristics of known motif,the 877 bp was divided into three fragments:-877~-1 bp,-637~-1 bp and-277~-1 bp.The three fragments were amplified and fused with GUS reporter gene to construct the fusion expression cassettes,and the three plant binary expression vectors were transformed into Arabidopsis thaliana Col-0 by flower dipping met-hod mediated.The results of GUS histochemical stainingshowed that the effective promoter of SFIBO gene was 637 bp DNA fragment upstream of the initiation codon,and the cis-acting element responsive toβ-ocimene inducation was located in the 360 bp DNA sequence between-637 bp and-277 bp.These results lay a good foundation for the next step to determine the specific sequence ofβ-ocimene response element and study the function of SFIBO gene.
作者
谭安琪
刘瑞琪
何礼庆
刘春林
阮颖
Tan Anqi;Liu Ruiqi;He Liqing;Liu Chunlin;Ruan Ying(Key Laboratory of Hunan Provincial on Crop Epigenetic Regulation and Development,Hunan Agricultural University,Changsha,410128;College of Biological Science and Technology,Hunan Agricultural University,Changsha,410128;College of Agronomy,Hunan Agricultural University,Changsha,410128)
出处
《分子植物育种》
CAS
北大核心
2022年第22期7309-7315,共7页
Molecular Plant Breeding
基金
国家自然科学基金面上项目(31771838)资助。
