三种材料修复大鼠骨质疏松性骨缺损的比较

Comparison of three materials for repairing osteoporotic bone defects in rats

[目的]评价磷酸钙骨水泥(calcium phosphate cement,CPC)/纤维蛋白胶(fibrin glue,FG)/力生长因子(mechanogrowth factor,MGF)/骨髓基质干细胞(bone marrow mesenchymal stem cells,BMSCs)组织工程人工骨修复大鼠骨质疏松性骨缺损的效果。[方法]体外构建CPC/FG(支架)、CPC/FG/BMSCs(支架细胞)和CPC/FG/MGF/BMSCs(全复合)材料。将54只Sprague-Dawley雌性大鼠随机分为3组,先行双卵巢切除,3个月后再次手术制造4 mm的桡骨缺损,分别植入CPC/FG、CPC/FG/BMSCs和CPC/FG/MGF/BMSCs。术后4周及8周行放射学、组织学染色、ALP免疫组化检测。[结果]与术后4周相比,术后8周3组动物影像和组织学评分均显著增加(P<0.05),而ALP免疫组化的IOD值显著下降(P<0.05)。相应时间点,影像评分、组织学评分和ALP免疫组化IOD值由高至低依次为,全复合组>支架细胞组>支架组,总体差异均有统计学意义(P<0.05)。[结论]与单纯支架或支架细胞材料相比,含力生长因子的全复合材料具有更好的修复骨质疏松性骨缺损的能力。

[Objective]To evaluate the efficacy of calcium phosphate cement(CPC)/fibrin glue(FG)/mechano-growth factor(MGF)/bone marrow mesenchymal stem cells(BMSCs)tissue engineered artificial bone in repairing osteoporotic bone defects in rats.[Methods]The CPC/FG(scaffold),CPC/FG/BMSCs(scaffold-cells)and CPC/FG/MGF/BMSCs(fully composed)were constructed in vitro.Fifty-four Sprague-Dawley female rats were randomly divided into 3 groups.The rats were firstly treated with bilateral ovariectomies,3 months later had a 4 mm radial bone defect in length created,then CPC/FG,CPC/FG/BMSCs and CPC/FG/MGF/BMSCs filled respectively.Radiographic check,histological observation and ALP immunohistochemistry were conducted 4 weeks and 8 weeks postoperatively.[Results]Compared with those at 4 weeks after operation,all the 3 groups of animals got significant increases in terms of radiographic and histological scores(P<0.05),whereas the IOD values of ALP immunohistochemistry decreased significantly at 8 weeks postoperatively(P<0.05).At all the corresponding time points,the radiographic and histological scores,as well as the ALP immunohistochemistry IOD value was all ranked up-down as:the fully composed group>scaffold cell group>scaffold group,with statistical significances in total(P<0.05).[Conclusion]Compared with the pure scaffold or scaffold-cell materials,fully composed material containing mechano-growth factor has better capacity to repair osteoporotic bone defect.