改良腹膜黏连大鼠动物模型的建立

Establishment and evaluation of an improved rat model of peritoneal adhesion

目的构建一种改良腹膜黏连大鼠动物模型。方法48只Sprague-Dawley(SD)大鼠完全随机均分为假手术对照组和实验组。假手术对照组仅做剖腹探查。实验组开展改良盲肠-腹膜黏连造模,通过摩擦1 cm×2 cm范围内盲肠浆膜造成损伤,同时刮擦对侧腹壁形成1 cm×2 cm缺损,术中评价盲肠损伤程度。术后3、7、30 d分别剖腹探查腹膜黏连程度,黏连组织行苏木精-伊红(HE)及马松(Masson)染色。结果该模型构建平均时间25 min,术中盲肠出血点平均为(11.670±0.271)个。与假手术空白组比较,实验组术后3、7、30 d剖腹探查均可见盲肠与腹膜黏连。HE及马松染色显示黏连带中大量胶原纤维沉积。结论该改良方法为构建腹膜黏连大鼠动物模型提供稳定工具。

Objective To establish an improved rat model of peritoneal adhesion.Methods 48 Sprague-Dawley rats were randomly divided into control group and experimental group.The rats in the control group only underwent exploratory laparotomy.In the experimental group,an improved rat model of cecum-abdominal wall adhesion was established by abrasing cecum serosa membrane within 1 cm×2 cm area and scraping the contralateral abdominal wall within 1 cm×2 cm area.The severity of cecum injury was evaluated during surgery.At the 3rd,7th and 30th day,the laparotomy was performed to evaluate the peritoneal adhesion and the adhesion area was measured.The histological evaluation of adhesion was performed by hematoxylin and eosin(HE)and Masson’s trichrome staining.Results The average operation time was 25 min,and the average number of intraoperative cecum bleeding spots were 11.670±0.271.Compared with the control group,cecum-abdominal wall adhesion was observed in the experimental group at the 3rd,7th and 30th day after operation.HE and Masson’s trichrome staining showed the formation of adhesion fusion and significant deposition of collagen fibers in the adhesion area.Conclusion This protocol provides a stable tool for developing rat model of peritoneal adhesion.