三叶青突变体的理化成分评价与分子鉴定

Physicochemical Composition Evaluation and Molecular Identification of Mutants of Tetrastigma hemsleyanum

为探究三叶青品种泽青1号是否产生药理成分突变,对26份三叶青样本的可溶性多糖、总酮、总皂苷、总酚、蛋白质、矿物质及游离氨基酸进行了理化评价与相关性分析,并检测了其ITS序列的位点突变情况。结果筛选出103、107、109、112、113、125、126、127、130、131、132等优异突变体。泽青1号突变体类群与其23个近缘崖爬藤属种间同源性为49%、平均遗传距离为0.54、组间距离为0.993;泽青1号突变体间同源性为93%~99%、配对距离为0.00640~0.11696、组内距离为0.06070,成功构建了基于ITS种属鉴定的突变体DNA条形码。本研究初步筛选出11个具有多元化定向育种应用价值的突变体,为解决三叶青种质资源稀缺、退化的问题提供了材料基础;同时基于ITS分子标记成功鉴定并区分泽青1号突变体类群与其他近缘崖爬藤属物种,筛选出突变类群的突变位点,为三叶青突变体种质资源评价提供了技术支撑。

In order to explore whether the mutation of Tetrastigma hemsleyanum Diels et Gilg(T.hemsleyanum)Zeqing 1 produced varied pharmacological component,the physicochemical quality such as soluble polysaccharides,total ketones,total saponins,total phenols,soluble proteins,minerals and free amino acids of 26 samples were evaluated and their correlation analysis were performed,and the mutated sites of ITS sequences of 26 samples were detected.The results showed that 11 excellent mutants 103,107,109,112,113,125,126,127,130,131,132 derived from Zeqing 1 were selected.The interspecific homology,overall mean distance,and between group mean distance among groups between Zeqing1 and its 23 closely related species were 49%,0.54,and 0.993 respectively.Intraspecific homology,pairwise distance and mean distance within groups among mutants were 93%~99%,0.00640~0.11696 and 0.06070 respectively.The DNA barcode of mutants based on ITS species identification was successfully constructed.In this study,11 mutants with application value of diversified directional breeding were screened which provide germplasm resources for solving the scarcity and degradation of T.hemsleyanum.Meanwhile,the mutant group derived from Zeqing 1 was successfully identified and distinguished from other related cliff climbing species based on ITS molecular markers,and the mutation sites of the mutant group were screened,which provided the technique support for the evaluation of germplasm resources of T.hemsleyanum mutants.