葡萄VvERF90的克隆及对乙烯合成基因的调控研究

Cloning of VvERF90 in Grape and Its Regulation on Ethylene Synthesis Gene

ERF转录因子为植物特有的一类转录因子,在调控植物乙烯合成过程中发挥着重要功能。为研究葡萄ERF转录因子在调控乙烯合成过程中的作用,以阳光玫瑰(Vitis labruscana Baily×V.vinifera L.)葡萄穗梗为材料,克隆了乙烯响应因子VvERF90,并进行了生物信息学分析和基因功能分析。结果表明,VvERF90属于第IX亚家族,与苹果MdERF2和MdERF3的亲缘关系较近;VvERF90可以正调控VvACO1的表达;VvERF90在拟南芥中过量表达后,转基因植株中VvACO1的同源基因AtACO3基因表达量明显升高,转基因植株的乙烯释放量增加2倍,植株变矮。上述结果表明,VvERF90可能通过调节VvACO1的表达,调控乙·烯的释放水平。本研究结果为进一步探明葡萄中乙烯合成的调控机制奠定了理论基础。

ERF transcription factors are unique to plants and play important roles in regulating ethylene synthesis.In order to explore the role of ERF transcription factor in regulating ethylene synthesis in grape,rachis of grape Shine Muscat(Vitis labruscana Baily×V.vinifera L.)was used as material ethylene response factor VvERF90 was cloned,and bioinformatics and functional analysis were carried out.The result shows that VvERF90 belongs to ERF IX subfamily,has close relationship with apple MdERF2 and MdERF3,and it positively regulate the expression of VvACO1 promoter.In the transgenic Arabidopsis thaliana plants overexpressed VvERF90 gene,expression of AtACO3,homologous gene VvACO1 was significantly increased,ethylene production was increased by two times,and height of transgenic Arabidopsis thaliana plants was shorter.The above results revealed that VvERF90 may modulate expression of VvACO1 to regulate ethylene production.This research lays foundation for studing ethylene synthesis mechanism in grape.