基于SSR标记的我国主栽日本栗品种(系)遗传结构分析和指纹图谱构建

Genetic Structure Analysis and Fingerprinting Construction of the Main Japanese Chestnut Cultivars(Lines)Using SSR Markers in China

为解析我国主栽日本栗品种资源的遗传多样性并构建其指纹图谱,本研究通过毛细管电泳和高质量的17个SSR分子标记对日本栗品种资源进行位点检测。结果显示,日本栗与茅栗具有较近的种间亲缘关系。在59份日本栗品种(系)中共检测到131个等位位点,每个标记平均有7.706个等位位点;多态信息含量(PIC)变幅为0.375~0.815,平均值为0.605;供试日本栗品种表现出高的遗传多样性。系统发育树、群体结构和主坐标分析结果一致,支持中国境内的部分日本栗品种(系)独立形成一个分支,且大多品种为杂交资源。此外,本研究根据多位点匹配分析确定了CmSI0922、CmSI00702和CmSI0658为核心引物,并利用这3个核心引物构建了59份日本栗品种(系)的指纹图谱。综上所述,相比其他栗属植物,日本栗分布范围较狭窄,但其具有丰富的遗传多样性,且品种资源间存在广泛的基因流。本研究成功构建了59份日本栗品种(系)资源的具有唯一对应关系的指纹图谱,为栗属植物的资源鉴定和保护利用提供了有力支撑。

In order to evaluate the genetic diversity of Japanese chestnut cultivar resources in China and construct their fingerprinting map,the loci of 17 high-quality fluorescent SSR markers were detected by capillary electrophoresis in this study.The results showed that Castanea crenata and Castanea seguinii have a relatively close relationship.A total of 131 alleles were detected from 59 Japanese chestnut cultivars,with an average of 7.706,polymorphism information content(PIC)was 0.375~0.815,with an average of 0.605,the tested Japanese chestnut cultivars(lines)showed rich genetic diversity.Phylogenetic tree,population structure and principal coordinate analysis consistently(PCoA)supported that part of Japanese chestnut cultivars(lines)in China formed an independent cluster,and most of the test cultivars(lines)were hybrids.According to the analysis of multi-locus matching,CmSI0922,CmSI00702 and CmSI0658 were identified the core primers.Then,a uniqu fingerprinting map of 59 Japanese chestnut cultivars(lines)was constructed with these three primers.In conclusion,although the distribution range of Japanese chestnuts were narrower than that of other chestnut plants,they had rich genetic diversity and there was extensive gene flow among Japanese chestnut cultivars.Meanwhile,the fingerprints of 59 Japanese chestnut cultivars(lines)resources with unique correspondence were successfully constructed with unique correspondence in this study,which will provide strong support for the resource identification,protection and utilization of chestnut plants.