褐斑病侵染下2,3-丁二醇-凹凸棒石复合制剂对草地早熟禾细胞壁组成物质及细胞壁降解酶活性的影响

Effect of 2,3-Butanediol-Palygorsk Compound Preparation on the Cell Wall Constituents and Cell Wall Enzyme Activities of Poa pratensis Infested by Brown Spot Disease

为了探究2,3-丁二醇(2,3-BD)与凹凸棒石(PAL)复合使用对植物诱抗能力的提升效果,以草地早熟禾纳苏(Poa pratensis Nassau)为试验材料,2,3-BD-PAL为复合诱导制剂,立枯丝核菌(Rhizoctonia solani)为接种病原物,对未诱导接菌、2,3-BD诱导接菌和2,3-BD-PAL复合诱导接菌后的植株叶片中细胞壁组成物质及细胞壁降解酶活性进行数据分析。结果表明,在病原菌胁迫期间,各处理组植株叶片中羧甲基纤维素酶(Cx)、β-葡萄糖苷酶(β-Glu)、滤纸酶(FPA)、果胶甲基半乳糖醛酸酶(PMG)和多聚半乳糖醛酸酶(PG)活性均显著升高,而多聚半乳糖醛酸反式消除酶(PGTE)和果胶甲基反式消除酶(PMTE)活性变化不明显,其中Cx起主要降解作用。在胁迫3 d时,未诱导叶片Cx、β-Glu和PG等酶活性快速上升到最大值,而诱导处理有效抑制了酶活的增加。草地早熟禾经2,3-BD-PAL复合诱导处理后,能够稳定纤维素含量,高效促进果胶合成和木质素积累,抑制菌丝入侵,更大程度上降低Cx、β-Glu和PG等降解酶对细胞壁的破坏程度,其诱抗效果优于2,3-BD单独诱导处理。综上,2,3-BD与PAL复合诱导能够增强2,3-BD的诱导效果,提高植物抗病性,为2,3-BD在农业生产中的广谱性应用奠定了理论基础。

In this experiment,in order to explore the effect of 2,3-butanediol(2,3-BD)combined with palygorsk(PAL)on improving the resistance of plants,Poa pratensis Nassau(Poa pratensis Nassau),2,3-BD-PAL and Rhizoctonia solani were used as the test material compound inducer and inoculate pathogens respectively.Through analyzing the cell wall composition and cell wall enzyme activities status in the plant leaves after uninduced inoculation,2,3-BD induced inoculation and 2,3-BD-PAL compound induced inoculation.The results showed that under pathogen stress,the activities of cellulose(Cx),β-glucosidase(β-Glu),filter paper en-zyme(FPA),pectin methyl-galacturonase(PMG)and polygalacturonase(PG)in leaves of treatment group were significantly increased,while the activities of polygalacturonic acid transeliminase(PGTE)and pectin methyl transeliminase(PMTE)did not change significantly.Among them,Cx plays a major role in degradation.Under stress for 3 days,the enzyme activities of Cx,β-Glu and PG in leaves of uninduced increased rapidly to the maximum value,but the increase of enzyme activities was effectively inhibited by induction treatment.After induction treatment with 2,3-BD-PAL,Poa pratensis can stabilize the cellulose content,efficiently promote pectin synthesis and lignin accumulation,inhibited the invasion of hyphae,and reduced the damage to the cell wall by degrading enzymes such as Cx,β-Glu and PG was to a greater extent,and its induction effect was better than that induced by 2,3-BD alone.In conclusion,the compound induction of 2,3-BD and PAL can enhance the induction effect of 2,3-BD and improve plant disease resistance,laying a theoretical foundation for its broad spectrum application in agricultural production.