光照对香菇菌丝后熟转色过程中活性氧代谢及自噬的影响

Effects of Light on Reactive Oxygen Species Content and Autophagy at Brown Mycelium Film Formation Stage in Lentinula edodes

为研究光照对香菇菌丝后熟转色过程中活性氧代谢的影响,通过设置自然光照条件与黑暗条件下香菇菌丝后熟转色对比试验,分析其活性氧含量、丙二醛(MDA)含量、还原型烟酰胺腺嘌呤二核苷酸磷酸(NADPH)氧化酶活性、抗氧化酶活性及自噬的差异。结果表明,自然光照条件下香菇菌丝正常后熟转色,黑暗条件下香菇菌丝不转色。自然光照条件下香菇菌丝活性氧含量、MDA含量、NADPH氧化酶活性、过氧化氢酶(CAT)活性、过氧化物酶(POD)活性均显著高于黑暗条件,超氧化物歧化酶(SOD)活性变化则与之相反。同时,香菇菌丝细胞自噬的透射电镜分析及实时荧光定量PCR(qRT-PCR)对自噬基因Atg8的表达水平分析结果表明,在自然光照条件下香菇菌丝细胞的自噬特征明显。本研究结果为优化香菇栽培的环境条件提供了一定的理论依据,也为后续对光照环境下香菇体内氧化平衡的作用机理研究提供了有效参考。

To further investigate the effect of light on brown mycelium film formation of Lentinula edodes,experiment were conducted under natural light and dark condition,then analyzing the changes of reactive oxygen species(ROS)content,malondialdehyde(MDA)content,NADPH oxidase activity,several antioxidant enzyme activity and autophagy.Results showed that the mycelium only developed brown film under natural light condition.Compared to dark conditions,ROS content,MDA content,NADPH oxidase activity,catalase(CAT)activity and peroxidase(POD)activity of L.edodes mycelium under light were significantly higher,while superoxide dismutase(SOD)activity was decreased.Also,transmission electron microscope and the expression level of autophagy gene Atg8 examined by real-time fluorescence quantitative PCR showed features of cell autophagy were more obvious in L.edodes mycelium under light.This research revealed the important role of light in brown mycelium film formation of L.edodes,and provides a theoretical basis for optimizing environmental conditions during cultivation of L.edodes,also provides a reference basis for further research on mechanism of oxidative balance in L.edodes under light.