基于灰色关联分析的芍药产地初加工条件优化

Condition optimization of original processing methods of Paeonia lactiflora Pall. using grey correlation analysis

以2020年版《中国药典》(一部)关于白芍、赤芍的产地初加工描述为依据,采用色彩色差分析法、高效液相色谱法(HPLC)分析芍药鲜根经不同产地初加工处理后的外观、断面色度值和芍药苷含量,并通过灰色关联分析方法对产地初加工条件进行优化。结果表明:去皮处理、冷(热)水煮处理、水煮时间、干燥方式均对芍药苷含量有较大影响,水煮处理对药材断面颜色和色度值亦有一定影响;灰色关联分析进一步证明芍药苷含量与干燥方式关联度最高(关联系数为0.834),其次是水煮时间;药材断面色度值与水煮时间的关联度较高,关联系数为0.746。推荐芍药鲜根产地初加工过程中以鲜根投入沸水中煮1~8 min、去皮、60℃烘干的方式获得白芍饮片;鲜根去杂后以60℃烘干的方式获得赤芍饮片。

This study was set to learn about the original processing methods of PAEONIAE RADIX ALBA(PRA) and PAEONIAE RADIX RUBRA(PRR) in the Part I of Chinese Pharmacopoeia(2020 version). We used color difference meters and high performance liquid chromatography(HPLC) methods to charaterize the appearance, chromatic value of section and content of paeoniflorin after processing the fresh roots. Then grey correlation analysis(GCA) was applied to optimize different original processing conditions. The results showed that the content of paeoniflorin was significantly affected by peeling, cold or hot water boiling, boiling time and drying method, and boiling treatment also had partly influence the sectional color and chromatic value. The GCA analysis further showed that the correlation degree between the content of paeoniflorin and the drying method was the highest(correlation coefficient was 0.834), followed by the boiling time, and the value of correlation coefficient between the section chromatic value and boiling time was 0.746.Thus, our study found that the optimal processing method should prepare boiling 1-8 min for the fresh root, peeling and then drying at 60 ℃ for PRA, and drying at 60 ℃ for PRR.