干旱胁迫下乙烯利对草地早熟禾叶绿素代谢基因表达的影响

Effect of Ethephon on Chlorophyll Metabolism Genes Expression of Kentucky Bluegrass under Drought Stress

在基因表达途径层面,分析乙烯利对干旱胁迫下草地早熟禾叶绿素代谢的调控作用。基于前期获得的已验证草地早熟禾Illumina Hiseq测序数据,以不同干旱胁迫处理下草地早熟禾叶片为材料,通过生物信息学筛选叶绿素合成和降解途径中的差异表达基因,并分析其不同处理下相关基因表达的差异情况,研究乙烯利对干旱胁迫下草地早熟禾叶绿素代谢基因表达的影响。结果表明:乙烯利预处理在一定程度上缓解干旱对叶绿素代谢合成降解途径的影响,具体表现为抑制由于干旱导致的叶绿素降解基因如PpPAO和PpNOL的大幅上调表达,此外干旱对血红素的合成也有一定程度的促进作用,间接减少了叶绿素合成前体物质,但乙烯利处理并未诱导血红素合成途径基因表达。本结果为解释乙烯利提升干旱胁迫下草地早熟禾叶绿素含量即叶片坪观质量的作用机理提供理论基础。

Based on the pre-acquired validated Illumina Hiseq sequencing data of Kentucky bluegrass,this study explored the effect of ethephon on chlorophyll metabolism of Kentucky bluegrass under drought stress,screening for differentially expressed genes in chlorophyll synthesis and degradation pathways by bioinformatics.The results showed that ethephon pretreatment alleviated the impact of drought stress on chlorophyll metabolism,synthesis and degradation pathways to a certain extent,specifically by inhibiting drought-induced up-regulation of chlorophyll degradation genes expression,promoting heme synthesis,and indirectly reducing chlorophyll synthesis precursors.However,ethephon treatment did not induce heme synthesis The study aimed provide a theoretical basis for explaining the mechanism of ethephon increasing chlorophyll content,that is,leaf turf quality of grassland precocious grass under drought stress.