牛磺酸复合添加剂对免疫低下小鼠免疫功能的影响

Effect of Taurine Additive Package on the Immune Function of Immunosuppressed Mice

【目的】研究在基础饲粮中添加牛磺酸复合添加剂对免疫低下模型小鼠免疫功能的影响,旨在为提高动物免疫机能的营养调控措施提供一定的理论依据。【方法】将80只小鼠随机均分为4组:C和M组小鼠饲喂基础饲粮,H和MH组小鼠给予含有8.8%的牛磺酸复合添加剂的基础饲粮,试验期51 d。M和MH组小鼠于试验第31天起连续4 d腹腔注射70μg/g BW环磷酰胺(CTX),制备免疫低下模型,其余小鼠腹腔注射等体积生理盐水。统计整个试验期的日采食量,每周称量小鼠体重,并计算平均日采食量(ADFI)和平均日增重(ADG)。试验结束后,采集小鼠血清及胸腺和脾脏,检测小鼠免疫器官指数、细胞免疫、体液免疫、非特异性免疫功能及血清中细胞因子和免疫球蛋白水平的变化。【结果】与C组相比,M组小鼠ADFI、耳肿胀度、血清溶血素水平、免疫球蛋白G(IgG)水平显著降低(P<0.05),ADG、胸腺指数、脾淋巴细胞增殖能力、抗体生成细胞量、腹腔巨噬细胞吞噬能力、白介素6(IL-6)和免疫球蛋白A(IgA)水平极显著降低(P<0.01),脾脏指数极显著升高(P<0.01);碳廓清指数和免疫球蛋白M(IgM)水平无显著差异(P>0.05);与M组相比,MH组小鼠的ADFI、ADG、胸腺指数、脾淋巴细胞增殖能力、耳肿胀度和IgM水平均显著提高(P<0.05),血清溶血素水平、抗体生成细胞量、腹腔巨噬细胞吞噬能力、IL-6和IgA水平均极显著提高(P<0.01),脾脏指数显著降低(P<0.05),碳廓清指数和IgG水平无显著差异(P>0.05)。【结论】该牛磺酸复合添加剂能够改善由CTX所致免疫低下小鼠的固有免疫和适应免疫,改善细胞因子和免疫球蛋白的水平,起到增强小鼠免疫力的作用。

【Objective】 This experiment was conducted to study the effect of adding taurine additive package in basic diet on the immune function of immunosuppressed mice.【Method】 80 mice were randomly and equally divided into four groups, mice in C and M groups were given basic diet, mice in H and MH groups were given diet containing 8.8% taurine additive package.The experiment was lasted for 51 days, the immunosuppressed mice model was established by intraperitoneal injection of 70 μg/g BW cyclophosphamide(CTX) for four consecutive days from the 31 st day(in M and MH groups),while the other mice were intraperitoneally injected with the equal volume of normal saline.The feed intake and body weight were recorded to calculate the average daily gain(ADG) and average daily feed intake(ADFI).Effects of this immunopotentiator on immune organ index, cellular immunity, humoral immunity, non-specific immune function, levels of cytokines and immunoglobulins of mice were detected.【Result】 Compared with C group, mice in M group showed significant reduction in ADFI,ear swelling, serum hemolysin level, and immunoglobulin G(IgG)(P<0.05),while ADG,thymus index, splenic lymphocyte proliferation capacity, antibody-producing cell amount, abdominal macrophage phagocytosis, interleukin-6(IL-6) and immunoglobulin A(IgA) levels were extremely significantly reduced(P<0.01),and the spleen index was extremely significantly increased(P<0.01).There were no significant differences in carbon clearance index and immunoglobulin M(IgM) level between C and M groups(P>0.05).Compared with M group, mice in MH group had significantly increased ADFI,ADG,thymus index, spleen lymphocyte proliferation, ear swelling and IgM levels(P<0.05),extremely significantly increased serum hemolysin level, antibody-producing cell amount, abdominal macrophage phagocytosis, IL-6 and IgA levels(P<0.01),and significantly decreased spleen index(P<0.05).There was no significant difference in carbon clearance index and IgG levels between M and MH group(P>0.05).【Conclusion】 The taurine additive package administered in the present study could improve the innate and adaptive immunity, improve the levels of cytokines and immunoglobulins of mice with low immunity caused by CTX,and play a role in enhancing the immunity of mice.