宫颈癌患者中VEGF、OLFM4、miR-124表达水平及联合对复发预测价值分析

Analysis of the expression levels of VEGF,OLFM4 and miR-124 in patients with cervical cancer and the value of their combination in predicting recurrence

目的分析嗅觉介导素4(olfactomedin,OLFM4)、血管内皮生长因子(vascular epithelial growth factor,VEGF)、微小RNA-124(microRNA-124,miR-124)检测在宫颈癌(cervical cancer,CC)、宫颈癌前病变(cervical intraepithelial neoplasia,CIN)中的表达情况及临床意义。方法选取于我院行手术治疗的82例CC标本为CC组,同期82例CIN标本为CIN组,82例正常宫颈组织标本为正常组,比较3组以及CC组不同病理参数患者组织标本中VEGF、OLFM4、miR-124表达情况,以Spearman相关性分析探讨组织各指标表达与CC组病理参数的关系,并观察不同预后(复发情况)CC患者组织标本中各指标表达,并分析复发预测价值。结果CC组VEGF、OLFM4阳性表达率均高于CIN组、正常组,miR-124表达水平均低于CIN组、正常组(P<0.05);不同分期、分化程度、复发情况VEGF、OLFM4、miR-124表达阳性率比较差异有统计学意义(P<0.05);VEGF、OLFM4、miR-124对复发的预测AUC分别为0.820、0.895、0.852,联合预测AUC为0.945,明显高于单一预测AUC。结论CC患者癌组织中VEGF、OLFM4阳性率明显升高,miR-124表达水平降低,各指标表达情况与CC生物学行为相关,可有效评估肿瘤增殖、侵袭,预测术后复发。

Objective To investigate the expression of olfactory mediator 4(OLFM4),vascular endothelial growth factor(VEGF),and microRNA-124(miR-124)in cervical cancer(CC)and cervical precancerous lesions(CIN)and their clinical significance.Methods A total of 82 specimens of patients with CC undergoing surgical treatment in our hospital were selected as the CC group,82 cases of CIN specimens were selected as the CIN group during the same period,and 82 cases of normal cervical tissue specimens were selected as the normal group.The 3 groups and the CC group were compared for VEGF,OLFM4,and miR-124 expression in tissue specimens of patients with different pathological parameters.Spearman correlation analysis was used to explore the relationship between the expression of various indicators in the tissues and the pathological parameters of the CC group,and the expression of various indicators in the tissue specimens of CC patients with different prognosis(recurrence)was observed;The value of various indicators in predicting the recurrence was analyzed.Results The positive expression rate of VEGF and OLFM4 in CC group was higher than that in CIN group and normal group,while the expression level of miR-124 was lower than that in CIN group and normal group(P<0.05).The positive rates of VEGF,OLFM4,and miR-124 expression were significantly different in patients with different stages,differentiation degree and recurrence(P<0.05).The area under the receiver operating characteristic(ROC)curve(AUC)of VEGF,OLFM4 and miR-124 in predicting the recurrence was 0.820,0.895 and 0.852,respectively,and the AUC of combined detection of VEGF,OLFM4 and miR-124 in prediction was 0.945,which was significantly higher than that of each indicator alone.Conclusion The positive rate of VEGF and OLFM4 in cancer tissues of CC patients is significantly higher and the expression level of miR-124 is lower.The expression of each indicator is associated with the biological behavior of CC,which could be used to effectively assess tumor proliferation and invasion and to predict postoperative recurrence.