T细胞受体γ位点反义RNA 1、人抗原R、三元基序家族蛋白21、缺氧诱导因子-1α在结直肠癌组织中的表达及临床意义

Expression and clinical significance of T cell receptor gamma locus antisense RNA 1,human antigen R,tripartite motif-containing protein 21 and hypoxia inducible factor-1αin colorectal cancer tissues

目的探讨T细胞受体γ位点反义RNA 1(TRG-AS1)、人抗原R(HuR)、三元基序家族蛋白21(TRIM21)、缺氧诱导因子-1α(HIF-1α)在结直肠癌组织中的表达及临床意义。方法收集103例结直肠癌患者的结直肠癌组织和癌旁组织,采用免疫组织化学染色法检测HuR、TRIM21、HIF-1α表达情况,采用实时定量逆转录聚合酶链反应(qRT-PCR)检测TRG-AS1相对表达量。比较结直肠癌组织和癌旁组织中TRG-AS1、HuR、TRIM21、HIF-1α的表达情况,分析TRG-AS1、HuR、TRIM21、HIF-1α表达情况与结直肠癌患者临床特征和预后的关系。结果结直肠癌组织中TRG-AS1相对表达量及HuR、HIF-1α阳性率均明显高于癌旁组织,TRIM21阳性率明显低于癌旁组织,差异均有统计学意义(P﹤0.01)。不同分化程度、淋巴结转移情况、血管侵犯情况结直肠癌患者结直肠癌组织中TRG-AS1相对表达量比较,差异均有统计学意义(P﹤0.05)。不同浸润深度、TNM分期、淋巴结转移情况结直肠癌患者结直肠癌组织中HuR、TRIM21、HIF-1α阳性率比较,差异均有统计学意义(P﹤0.05)。至随访结束,TRG-AS1低表达患者的累积生存率为55.6%,高于TRG-AS1高表达患者的31.5%(P﹤0.05);HuR阴性患者的累积生存率为56.9%,高于HuR阳性患者的37.0%(P﹤0.05);TRIM21阴性患者的累积生存率为22.4%,低于TRIM21阳性患者的59.0%(P﹤0.05);HIF-1α阴性患者的累积生存率为72.0%,高于HIF-1α阳性患者的36.6%(P﹤0.05)。结论结直肠癌组织中TRG-AS1、HIF-1α及HuR表达均升高,TRIM21表达降低,TRG-AS1、HuR、TRIM21、HIF-1α表达情况与结直肠癌侵袭、转移及患者预后密切相关,对评估结直肠癌患者病情及预后具有一定的临床价值。

Objective To investigate the expression and clinical significance of T cell receptor gamma locus antisense RNA 1(TRG-AS1),human antigen R(HuR),tripartite motif-containing protein 21(TRIM21)and hypoxia inducible factor-1α(HIF-1α)in colorectal cancer tissues.Method The colorectal cancer tissues and paracancerous tissues of103 colorectal cancer patients were collected.The expression of HuR,TRIM21,and HIF-1αwere detected by immunohistochemical staining.The relative expression of TRG-AS1 was detected by real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR).The expression of TRG-AS1,HuR,TRIM21,and HIF-1αin colorectal cancer tissues and paracancerous tissues were compared.The relationship between the expression of TRG-AS1,HuR,TRIM21,HIF-1αand the clinical characteristics and prognosis of colorectal cancer patients were analyzed.Result The relative expression of TRG-AS1 and the positive rate of HuR and HIF-1αin colorectal cancer tissues were significantly higher than those in paracancerous tissues,the positive rate of TRIM21 was significantly lower than that of paracancerous tissues,and the difference was statistically significant(P<0.01).There were statistically significant differences in the relative expression of TRG-AS1 in colorectal cancer tissues of colorectal cancer patients with different differentiation degrees,lymph node metastasis,and vascular invasion(P<0.05).There were statistically significant differences in the positive rate of HuR,TRIM21,and HIF-1αin colorectal cancer tissues of colorectal cancer patients with different invasion depths,TNM stages,and lymph node metastasis(P<0.05).At the end of follow-up,the cumulative survival rate of patients with low expression of TRG-AS1 was 55.6%,higher than 31.5%of patients with high expression of TRG-AS1(P<0.05);the cumulative survival rate of HuR-negative patients was 56.9%,higher than 37.0%of HuR-positive patients(P<0.05);the cumulative survival rate of TRIM21-negative patients was 22.4%,lower than 59.0%of TRIM21-positive patients(P<0.05);the cumulative survival rate of HIF-1α-negative patients was 72.0%,higher than 36.6%of HIF-1α-positive patients(P<0.05).Conclusion The expression of TRG-AS1,HIF-1α,and HuR were all increased,while the expression of TRIM21 was decreased in colorectal cancer tissues.The expression of TRG-AS1,HuR,TRIM21,and HIF-1αare closely related to the invasion,metastasis,and prognosis of colorectal cancer patients.These indicators have certain clinical value in evaluating the condition and prognosis of patients with colorectal cancer.