丁酸钠对染氟小鼠骨矿含量及骨微结构的影响

Effects of sodium butyrate on bone mineral content and bone microstructure in mice treated with sodium fluoride

目的探讨丁酸钠对氟中毒小鼠骨矿含量及骨微结构的影响。方法24只雄性ICR初断乳小鼠,按照体重均衡的原则分为对照组、氟中毒组、丁酸钠组。氟中毒组和丁酸钠组小鼠饮用含有100 mg/L氟化钠的蒸馏水,对照组小鼠自由饮水;从第14周开始,丁酸钠组小鼠按500 mg/kg丁酸钠灌胃,对照组和氟中毒组则以生理盐水灌胃;第20周结束后,取各组小鼠左侧胫骨和股骨,进行骨结构强度、Micro-CT及骨矿含量检测。结果与对照组比较,氟中毒组小鼠的骨小梁骨密度、骨体积分数、骨小梁数目、骨钙、骨镁、骨锌含量及骨结构强度均显著降低(t=4.921,P<0.01;t=6.959,P<0.01;t=4.915,P<0.05;t=2.363,P<0.05;t=6.553,P<0.01;t=2.461,P<0.05;t=4.166,P<0.01),而骨小梁间隙、骨小梁结构模型指数、骨氟、骨磷含量明显增高(t=-3.490,P<0.05;t=-3.820,P<0.05;t=-8.608,P<0.01;t=-8.641,P<0.01),骨小梁结构稀疏、粗细不均,呈现明显的骨微结构破坏。丁酸钠组小鼠骨小梁骨密度、骨钙、骨镁、骨锌含量及骨结构强度均显著高于氟中毒组(t=-3.557,P<0.05;t=-2.444,P<0.05;t=-3.271,P<0.01;t=-4.810,P<0.01;t=-2.552,P<0.05),骨氟和骨磷含量也明显降低(t=4.042,P<0.01;t=2.424,P<0.05),骨小梁结构相对紧密,结构破坏程度相对较轻。结论丁酸钠可以缓解氟中毒小鼠的骨代谢紊乱状态,改善骨小梁微结构,增强骨结构强度。

Objective To investigate the effect of sodium butyrate on bone mineral content and bone microstructure in mice with fluorosis.Methods According to the principle of weight balance,24 male ICR weaned mice were divided into control group,fluorosis group and sodium butyrate group.The mice in the fluorosis group and sodium butyrate group drank distilled water containing 100 mg/L sodium fluoride,and those in the control group drank water freely.At week 14,the mice in the sodium butyrate group were given 500 mg/kg sodium butyrate by gavage,while those in the control group and fluorosis group were given normal saline.After 20 weeks,the left tibia and femur of the mice in all the groups were removed,which were subjected to bone structure strength measurements,Micro-CT scanning and bone mineral content measurement.Results Compared with the control group,the trabecular bone mineral density,bone volume fraction,bone trabecular number,bone calcium,bone magnesium,bone zinc content and bone structure strength of the mice in the fluorosis group were all significantly decreased(t=4.921,P<0.01;t=6.959,P<0.01;t=4.915,P<0.05;t=2.363,P<0.05;t=6.553,P<0.01;t=2.461,P<0.05;t=4.166,P<0.01,respectively),while the trabecular separation,trabecular structure model index,bone fluoride and bone phosphorus content were all significantly increased(t=-3.490,P<0.05;t=-3.820,P<0.05;t=-8.608,P<0.01;t=-8.641,P<0.01,respectively).The bone CT results showed that the trabecular structure was sparse and irregular,and the bone micro-structures were damaged obviously.The bone trabecular,bone mineral density,bone calcium,bone magnesium,bone zinc content and bone structure strength of the mice in the sodium butyrate group were all significantly higher than those in the fluorosis group(t=-3.557,P<0.05;t=-2.444,P<0.05;t=-3.271,P<0.01;t=-4.810,P<0.01;t=-2.552,P<0.05,respectively),while the bone fluoride and bone phosphorus content was also significantly reduced(t=4.042,P<0.01;t=2.424,P<0.05,respectively).Our results showed that the structure of trabecular bone was relatively tight,and the degree of structural damage was relatively mild.Conclusion Sodium butyrate can alleviate the disorder of bone metabolism in mice with fluorosis,improve the microstructure of trabecular bone,and enhance the bone strength.