基于生物学信息探讨KIF26B在上皮性卵巢癌中的表达及临床意义

Expression and clinical significance of KIF26B in epithelial ovarian cancer based on biological information

目的探讨KIF26B基因在上皮性卵巢癌(EOC)中的表达并分析其与临床病理特征、肿瘤微环境免疫细胞浸润及预后的关系。方法下载TCGA(The Cancer Genome Atlas)数据库中EOC患者的临床信息和转录组数据,通过比例风险模型(Cox)、基因集富集(GSEA)、CIBERSORT、KM曲线等分析KIF26B与EOC临床病理特征、相关信号通路、微环境免疫细胞浸润、生存预后等相关关系,同时通过免疫组织化学对KIF26B在EOC中的表达进行验证。结果KIF26B mRNA在EOC中的表达显著高于正常卵巢组织,差异有统计学意义(P<0.05)。KIF26B表达与FIGO分期、肿瘤残余灶(RD)、不良预后、微环境免疫浸润细胞构成比例密切相关(P<0.05),与年龄、病理分级、是否手术治疗无关(P>0.05)。通路富集显示KIF26B参与间充质细胞分化、血管内皮生长因子(VEGF)、黏着斑等促进肿瘤复发转移的信号通路。多因素Cox分析显示KIF26B高表达是影响患者预后的独立危险因素(P<0.05),免疫组织化学进一步验证了KIF26B在EOC中高表达。结论KIF26B在EOC中高表达是影响患者预后的独立危险因素,且与肿瘤免疫浸润和EOC复发转移相关。KIF26B有望作为EOC预后标志物和潜在治疗靶点。

Objective To explore the expression of KIF26B gene in epithelial ovarian cancer(EOC)and to analyze the association of KIF26B withclinic-pathological features,immune cell infiltration in the tumor microenvironment and prognosis.Methods Clinical information and transcriptome data of EOC patients from TCGA(The Cancer Genome Atlas)database were collected.Theassociation of KIF26B with EOC clinicopathological features,related signaling pathways,microenvironmental immune cell infiltration,and survival prognosis was analyzed by proportional risk model(Cox),gene set enrichment(GSEA),CIBERSORT,and KM curves.KIF26B expression in EOC by immunohistochemistry was verified.Results KIF26B m RNA expression in EOC was significantly higher than that of the normal ovarian tissue(P<0.05).KIF26B expression was significantly correlated with FIGO stage,tumor residual foci(RD),poor prognosis,and the proportion of immune infiltrating cells in the microenvironment(P<0.05),independent of age,pathological grade,and whether postoperative(P>0.05).Pathway enrichment showed that KIF26B was involved in mesenchymal cell differentiation,vascular endothelial growth factor(VEGF),focal adhesion and other signaling pathways that promote tumor recurrence and metastasis.Multifactorial Cox analysis showed that high KIF26B expression was an independent risk factor for patient prognosis(P<0.05),and immunohistochemistry further validated that high KIF26B expression in EOC.Conclusion High expression of KIF26B in EOC was an independent risk factor for prognosis and was associated with tumor immune infiltration and EOC recurrence and metastasis.It is assumed that KIF26B could be a prognostic marker and potential therapeutic target for EOC.